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LearnPA5-21787
antibody from Invitrogen Antibodies
Targeting: CHMP5
C9orf83, CGI-34, HSPC177, SNF7DC2, Vps60
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-21787 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHMP5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse stomach. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (92%), Pig (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHMP5 using 50 µg of mouse stomach lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CHMP5 polyclonal antibody (Product # PA5-21787) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CHMP5 Polyclonal Antibody (Product # PA5-21787). Sample (30 µg whole cell lysate). A: 293T. B: A431. C: H1299. D: HeLa S3. E: Hep G2. 12% SDS PAGE. CHMP5 Polyclonal Antibody (Product # PA5-21787) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CHMP5 Polyclonal Antibody (Product # PA5-21787). Jurkat whole cell extracts and Jurkat exosome extract (15.4 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 Polyclonal Antibody (Product # PA5-21787) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CHMP5 Polyclonal Antibody detects CHMP5 protein at cytoplasm by immunofluorescent analysis. Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 Polyclonal Antibody (Product # PA5-21787) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CHMP5 was performed in paraffin-embedded mouse urinary bladder tissue using CHMP5 Polyclonal Antibody (Product # PA5-21787) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5 (Product # PA5-21787) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
