- PA5-28240 - Invitrogen Antibodies SLC5A1 antibody

Ng KM, Lau YM, Dhandhania V, Cai ZJ, Lee YK, Lai WH, Tse HF, Siu CW
Scientific reports 2018 Oct 5;8(1):14872

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SGLT1 using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SGLT1 polyclonal antibody (Product # PA5-28240) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SGLT1 Polyclonal Antibody (Product # PA5-28240). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 Polyclonal Antibody (Product # PA5-28240) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SGLT1 in methanol-fixed A431 cells using a SGLT1 polyclonal antibody (Product # PA5-28240) at a 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Sodium/glucose cotransporter 1 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SGLT1 Polyclonal Antibody (Product # PA5-28240) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing punctate vesicular localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Sodium/glucose cotransporter 1 was achieved by transfecting Caco-2 cells with Sodium/glucose cotransporter 1 specific siRNA (Silencer® select Product # S12952, S12953). Immunofluorescence analysis was performed on untransfected Caco-2 cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Sodium/glucose cotransporter 1 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with SGLT1 Polyclonal Antibody (Product # PA5-28240, 1:100 dilution) followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (Red) staining. Decrease in the vesicular punctate specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to Sodium/glucose cotransporter 1 (Green). The Images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human lung fibrose tissue, using SGLT1 (Product # PA5-28240) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Expression of SGLT1 and SGLT2 in hiPSC-derived cardiomyocytes and human heart tissue. ( A ) Confirmation of the identity of the PCR product obtained from SGLT2 -specific amplifications by DNA sequencing analysis. (B , C) Expression of SGLT1 and SGLT2 in human heart tissue were confirmed by PCR analysis and DNA sequencing analysis. (D) Protein levels of SGLT1 and SGLT2 in the hiPSC-derived cardiomyocytes were evaluated by Western blot analysis. *p < 0 . 05 . For the full-length images of gels and blots shown in this figure, please refer to Supplementary Figs S4 and S5 .

PA5-28240