Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3008 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Wnt-7a/b Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Wnt-7a and Wnt-7b in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-9a, rmWnt-11, and rmWnt-16 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation.
CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of β-catenin in carcinoma cells.
Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Heo WD, Kim DW, Yeo CY, Kim CH, Liu QS
Nature communications 2018 Aug 24;9(1):3434
Nature communications 2018 Aug 24;9(1):3434
WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation.
Rider V, Talbott A, Bhusri A, Krumsick Z, Foster S, Wormington J, Kimler BF
The Journal of endocrinology 2016 May;229(2):197-207
The Journal of endocrinology 2016 May;229(2):197-207
CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of β-catenin in carcinoma cells.
Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K
International journal of oncology 2012 Dec;41(6):2021-8
International journal of oncology 2012 Dec;41(6):2021-8
Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
Gogolla N, Galimberti I, Deguchi Y, Caroni P
Neuron 2009 May 28;62(4):510-25
Neuron 2009 May 28;62(4):510-25
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Wnt-7a/b in Human endometrial Cancer Tissue. Wnt-7a/b was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using 15 µg/mL Goat Anti-Human Wnt-7a/b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3008) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
