PA5-80231

antibody from Invitrogen Antibodies

Targeting: WNT7A Wnt-7a

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80231

Provider

Invitrogen Antibodies

Product name

WNT7A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Selective Surface and Intraluminal Localization of Wnt Ligands on Small Extracellular Vesicles Released by HT-22 Hippocampal Neurons.

Torres VI, Barrera DP, Varas-Godoy M, Arancibia D, Inestrosa NC
Frontiers in cell and developmental biology 2021;9:735888

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 2 Wnt ligand expression levels in HT-22 cells. (A) Comparison of western blot analysis of Wnt3a, Wnt5a, Wnt7a, and GPR177 protein content in cell lysate and P100 fraction. Each lane was loaded with 30 mug protein. (B) Summary densitometric measurement of band intensity showing enrichment (fold) of each protein in the P100 pellet relative to a corresponding lysate. Wnt3a, 0.4 +- 0.19 ( n = 5); Wnt5a, 8.70 +- 4.14 ( n = 7); Wnt7a, 0.22 +- 0.21 ( n = 4; four independent cell culture preparations); GPR177 3.30 +- 1.93 ( n = 3; independent cell culture preparations).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 5 Effects of Wnt-C59 on the content of Wnt ligands in the P100 fraction. (A) Comparison of the western blot analysis of Wnt3a, Wnt5a, Wnt7a, and GPR177 protein expression levels in the P100 fraction derived from control and Wnt-C59 treated cells (each lane was loaded with 30 mug protein). (B) Relative protein expression levels of Wnt ligands and GPR177 in the P100 fraction. The staining intensity of each band derived from control P100 was assigned a value of 100%, and the value obtained in the P100 from Wnt-C59 treatment was compared with its respective control. Wnt3a (Control: 100%, Wnt-C59: 51.1 +- 21.5%; n = 3; p = 0.0039); Wnt5a (Control: 100%, Wnt-C59: 39.1 +- 45.6%; n = 3; p = 0.0404); Wnt7a (Control: 100%, Wnt-C59: 74.82 +- 13.1%; n = 3; p = 0.0291); GPR177 (Control: 100%, Wnt-C59: 31.9 +- 13.6%; n = 3; p = 0.001). (C) Protein expression levels of Wnt ligands and GPR177 relative to the number of particles loaded per well. Control P100 was assigned a value of 100%, and the value obtained in the P100 from Wnt-C59 treatment was compared with its respective control. In both types of analysis of (B,C) , the Wnt3a, Wnt5a, Wnt7a, and GPR177 contents significantly decreased in the exosomes secreted by Wnt-C59 treated cells relative to their levels in the control cells based on the results of Mann-Whitney non-parametric t -student test (ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.005). Wnt3a (Control: 100%, Wnt-C59: 52.4 +- 24.9%; n = 3; p = 0.0295); Wnt5a (C