PA5-28289

antibody from Invitrogen Antibodies

Targeting: WNT7A Wnt-7a

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28289

Provider

Invitrogen Antibodies

Product name

WNT7A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: WNT7A-transfected 293T.

Concentration

0.21 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WNT7A using 30 µg of A) Non-transfected 293T lysates and B) WNT7A transfected 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a WNT7A polyclonal antibody (Product # PA5-28289) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of WNT7A was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNT7A Polyclonal Antibody (Product # PA5-28289) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using WNT7A Polyclonal Antibody (Product # PA5-28289). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT7A Polyclonal Antibody (Product # PA5-28289) diluted at 1:5,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WNT7A antibody detects WNT7A protein at secreted on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. WNT7A antibody (Product # PA5-28289) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WNT7A antibody detects WNT7A protein at secreted on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. WNT7A antibody (Product # PA5-28289) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.