Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-28289 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT7A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: WNT7A-transfected 293T. Predicted reactivity: Mouse (100%), Rat (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.21 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WNT7A using 30 µg of A) Non-transfected 293T lysates and B) WNT7A transfected 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a WNT7A polyclonal antibody (Product # PA5-28289) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WNT7A was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNT7A Polyclonal Antibody (Product # PA5-28289) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WNT7A Polyclonal Antibody (Product # PA5-28289). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT7A Polyclonal Antibody (Product # PA5-28289) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WNT7A antibody detects WNT7A protein at secreted on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. WNT7A antibody (Product # PA5-28289) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WNT7A antibody detects WNT7A protein at secreted on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. WNT7A antibody (Product # PA5-28289) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
