Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F41649 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ID1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 66-93 from the human protein was used as the immunogen for this ID1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the ID1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HepG2, MCF-7, U-2OS cell line (left to right) using ID1 antibody; Ab was diluted at 1:1000 for each lane.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ID1 antibody western blot analysis in mouse heart tissue lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ID1 antibody with U-251MG cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ID1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ID1 antibody flow cytometric analysis of U251 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.