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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00003397-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003397-M02, RRID:AB_539731
- Product name
- Mouse Monoclonal ID1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ID1 - inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ID1 Antibody (1F7) [H00003397-M02] - Western Blot analysis of ID1 expression in Y-79 ( Cat # L042V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ID1 Antibody (1F7) [H00003397-M02] - Analysis of ID1 over-expressed 293 cell line, cotransfected with ID1 Validated Chimera RNAi ( Cat # H00003397-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID1 monoclonal antibody (M02), clone 1F7 (Cat # H00003397-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ID1 Antibody (1F7) [H00003397-M02] - Analysis of ID1 expression in transfected 293T cell line by ID1 monoclonal antibody (M02), clone 1F7.Lane 1: ID1 transfected lysate(16.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ID1 Antibody (1F7) [H00003397-M02] - Detection limit for recombinant GST tagged ID1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ID1 Antibody (1F7) [H00003397-M02] - Analysis of monoclonal antibody to ID1 on HeLa cell. Antibody concentration 10 ug/ml.
