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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- M00945 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Id1 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for ID1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat ID1 information: Molecular Weight: 16133 MW; Subcellular Localization: Cytoplasm . Nucleus.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- ACC-9
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Regorafenib inhibits migration, invasion, and vasculogenic mimicry of hepatocellular carcinoma via targeting ID1-mediated EMT.
BMP-Id pathway targeted by cholesterol myristate suppresses the apoptosis of PC12 cells.
Zhang N, Zhang S, Wu W, Lu W, Jiang M, Zheng N, Huang J, Wang L, Liu H, Zheng M, Wang J
Molecular carcinogenesis 2021 Feb;60(2):151-163
Molecular carcinogenesis 2021 Feb;60(2):151-163
BMP-Id pathway targeted by cholesterol myristate suppresses the apoptosis of PC12 cells.
Chen DF, Cao JH, Liu Y, Wu Y, Du SH, Li H, Zhou JH, Li YW, Zeng HP, Hua ZC
Brain research 2011 Jan 7;1367:33-42
Brain research 2011 Jan 7;1367:33-42
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney, using Id1 Antibody(M00945)ID1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ID1 Antibody (M00945)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
