Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN952815 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1) (AA 73-101), (Middle Region) antibody
- Antibody type
- Polyclonal
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 73-101, Middle Region
- Vial size
- 0.4 mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references The role of inhibitor of DNA-binding (Id1) in hyperproliferation of keratinocytes: the pathological basis for middle ear cholesteatoma from chronic otitis media.
Id1 enhances RING1b E3 ubiquitin ligase activity through the Mel-18/Bmi-1 polycomb group complex.
Hamajima Y, Komori M, Preciado DA, Choo DI, Moribe K, Murakami S, Ondrey FG, Lin J
Cell proliferation 2010 Oct;43(5):457-63
Cell proliferation 2010 Oct;43(5):457-63
Id1 enhances RING1b E3 ubiquitin ligase activity through the Mel-18/Bmi-1 polycomb group complex.
Qian T, Lee JY, Park JH, Kim HJ, Kong G
Oncogene 2010 Oct 28;29(43):5818-27
Oncogene 2010 Oct 28;29(43):5818-27
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ID1 / BHLHB24 Antibody (Center) Cat.-No AP52144PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- IHC
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS