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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Other assay [1]
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- Product number
- A303-039A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- E2F8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human E2F8 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody (Product # A303-039A; lot A303-039A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human E2F8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F8 antibody A303-039A (lot A303-039A-2) used for IP at 3 µg per reaction. E2F8 was also immunoprecipitated by rabbit anti-E2F8 antibodies BL11013 and BL11014. For blotting immunoprecipitated E2F8, A303-039A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse E2F8 by western blot. Samples: Whole cell lysate (15 µg) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody A303-039A (lot A303-039A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human E2F8 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody A303-039A (lot A303-039A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human E2F8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F8 antibody (Product # A303-039A; lot A303-039A-3) used for IP at 6 µg per reaction. E2F8 was also immunoprecipitated by a previous lot of this antibody (lot A303-039A-2). For blotting immunoprecipitated E2F8, Product # A303-039A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
