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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-92143 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STCH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MPVANAVISV PAEFDLKQRN STIEAANLAG LKILRVINEP TAAAMAYGLH KADVFHVLVI DLGGGTLDVS LLNKQGGMFL TRAMSGNNKL GGQDFNQRLL QYLYKQIYQT YGFVPSRKEE IHRLRQAVEM VKLNLTLHQS AQLSVLLTVE EQDRKEPHSS DTELPKDKLS SADDHRVNSG FGRGLSDKKS GESQVLFETE ISRKLFDTLN EDLFQKILVP IQQVLKEGHL EKTEIDEVVL VGGSTRIPRI RQVIQEFFGK DPNTSVDPDL AVVTGVAIQA GIDGGSWPLQ VSALEIPNKH LQKTNFN; Positive Samples: U-87MG; Cellular Location: Endoplasmic reticulum, Microsome
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.95 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of U-87MG cells, using HSPA13 Polyclonal antibody (Product # PA5-92143) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of STCH in extracts of U-87MG cells using STCH Polyclonal Antibody (Product # PA5-92143) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of STCH was performed in C6 cells using STCH Polyclonal Antibody (Product # PA5-92143) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of STCH was performed in L929 cells using STCH Polyclonal Antibody (Product # PA5-92143) at a dilution of 1:100. Blue: DAPI for nuclear staining.
