Antibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- 13049-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13049-1-AP, RRID:AB_2059000
- Product name
- ARL8B antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ARL8B antibody (Cat. #13049-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Macrophage-derived MMP12 promotes fibrosis through sustained damage to endothelial cells.
DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy.
ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization.
Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes.
A program of successive gene expression in mouse one-cell embryos.
Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation.
RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.
Parkinson disease-associated Leucine-rich repeat kinase regulates UNC-104-dependent axonal transport of Arl8-positive vesicles in Drosophila.
The Salmonella effector SifA initiates a kinesin-1 and kinesin-3 recruitment process mirroring that mediated by Arl8a and Arl8b.
Specific KIF1A-adaptor interactions control selective cargo recognition.
mTORC1 activity is supported by spatial association with focal adhesions.
Lipid-mediated motor-adaptor sequestration impairs axonal lysosome delivery leading to autophagic stress and dystrophy in Niemann-Pick type C.
ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1.
Endosomal sorting drives the formation of axonal prion protein endoggresomes.
The GTPase Arl8B Plays a Principle Role in the Positioning of Interstitial Axon Branches by Spatially Controlling Autophagosome and Lysosome Location.
The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.
Hepatitis C virus infection increases autophagosome stability by suppressing lysosomal fusion through an Arl8b-dependent mechanism.
Neuronal Soma-Derived Degradative Lysosomes Are Continuously Delivered to Distal Axons to Maintain Local Degradation Capacity.
BORC/kinesin-1 ensemble drives polarized transport of lysosomes into the axon.
The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.
Salmonella exploits Arl8B-directed kinesin activity to promote endosome tubulation and cell-to-cell transfer.
Lysosomal positioning coordinates cellular nutrient responses.
Zhou X, Zhang C, Yang S, Yang L, Luo W, Zhang W, Zhang X, Chao J
Journal of hazardous materials 2024 Jan 5;461:132733
Journal of hazardous materials 2024 Jan 5;461:132733
DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy.
Kumar R, Khan M, Francis V, Aguila A, Kulasekaran G, Banks E, McPherson PS
Nature communications 2024 Jan 31;15(1):919
Nature communications 2024 Jan 31;15(1):919
ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization.
Menon D, Bhapkar A, Manchandia B, Charak G, Rathore S, Jha RM, Nahak A, Mondal M, Omrane M, Bhaskar AK, Thukral L, Thiam AR, Gandotra S
Cell reports 2023 Oct 31;42(10):113203
Cell reports 2023 Oct 31;42(10):113203
Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes.
Schleinitz A, Pöttgen LA, Keren-Kaplan T, Pu J, Saftig P, Bonifacino JS, Haas A, Jeschke A
Cell reports 2023 Jan 31;42(1):111969
Cell reports 2023 Jan 31;42(1):111969
A program of successive gene expression in mouse one-cell embryos.
Asami M, Lam BYH, Hoffmann M, Suzuki T, Lu X, Yoshida N, Ma MK, Rainbow K, Gužvić M, VerMilyea MD, Yeo GSH, Klein CA, Perry ACF
Cell reports 2023 Feb 28;42(2):112023
Cell reports 2023 Feb 28;42(2):112023
Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation.
Bauer CS, Webster CP, Shaw AC, Kok JR, Castelli LM, Lin YH, Smith EF, Illanes-Álvarez F, Higginbottom A, Shaw PJ, Azzouz M, Ferraiuolo L, Hautbergue GM, Grierson AJ, De Vos KJ
Frontiers in cellular neuroscience 2022;16:1061559
Frontiers in cellular neuroscience 2022;16:1061559
RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.
Keren-Kaplan T, Sarić A, Ghosh S, Williamson CD, Jia R, Li Y, Bonifacino JS
Nature communications 2022 Mar 21;13(1):1506
Nature communications 2022 Mar 21;13(1):1506
Parkinson disease-associated Leucine-rich repeat kinase regulates UNC-104-dependent axonal transport of Arl8-positive vesicles in Drosophila.
Inoshita T, Liu JY, Taniguchi D, Ishii R, Shiba-Fukushima K, Hattori N, Imai Y
iScience 2022 Dec 22;25(12):105476
iScience 2022 Dec 22;25(12):105476
The Salmonella effector SifA initiates a kinesin-1 and kinesin-3 recruitment process mirroring that mediated by Arl8a and Arl8b.
Fang Z, Fallet M, Moest T, Gorvel JP, Méresse S
Journal of cell science 2022 Dec 1;135(1)
Journal of cell science 2022 Dec 1;135(1)
Specific KIF1A-adaptor interactions control selective cargo recognition.
Hummel JJA, Hoogenraad CC
The Journal of cell biology 2021 Oct 4;220(10)
The Journal of cell biology 2021 Oct 4;220(10)
mTORC1 activity is supported by spatial association with focal adhesions.
Rabanal-Ruiz Y, Byron A, Wirth A, Madsen R, Sedlackova L, Hewitt G, Nelson G, Stingele J, Wills JC, Zhang T, Zeug A, Fässler R, Vanhaesebroeck B, Maddocks ODK, Ponimaskin E, Carroll B, Korolchuk VI
The Journal of cell biology 2021 May 3;220(5)
The Journal of cell biology 2021 May 3;220(5)
Lipid-mediated motor-adaptor sequestration impairs axonal lysosome delivery leading to autophagic stress and dystrophy in Niemann-Pick type C.
Roney JC, Li S, Farfel-Becker T, Huang N, Sun T, Xie Y, Cheng XT, Lin MY, Platt FM, Sheng ZH
Developmental cell 2021 May 17;56(10):1452-1468.e8
Developmental cell 2021 May 17;56(10):1452-1468.e8
ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1.
Keren-Kaplan T, Bonifacino JS
Current biology : CB 2021 Feb 8;31(3):540-554.e5
Current biology : CB 2021 Feb 8;31(3):540-554.e5
Endosomal sorting drives the formation of axonal prion protein endoggresomes.
Chassefeyre R, Chaiamarit T, Verhelle A, Novak SW, Andrade LR, Leitão ADG, Manor U, Encalada SE
Science advances 2021 Dec 24;7(52):eabg3693
Science advances 2021 Dec 24;7(52):eabg3693
The GTPase Arl8B Plays a Principle Role in the Positioning of Interstitial Axon Branches by Spatially Controlling Autophagosome and Lysosome Location.
Adnan G, Rubikaite A, Khan M, Reber M, Suetterlin P, Hindges R, Drescher U
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Oct 14;40(42):8103-8118
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Oct 14;40(42):8103-8118
The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.
Lu M, van Tartwijk FW, Lin JQ, Nijenhuis W, Parutto P, Fantham M, Christensen CN, Avezov E, Holt CE, Tunnacliffe A, Holcman D, Kapitein L, Schierle GSK, Kaminski CF
Science advances 2020 Dec;6(51)
Science advances 2020 Dec;6(51)
Hepatitis C virus infection increases autophagosome stability by suppressing lysosomal fusion through an Arl8b-dependent mechanism.
Jones-Jamtgaard KN, Wozniak AL, Koga H, Ralston R, Weinman SA
The Journal of biological chemistry 2019 Sep 27;294(39):14257-14266
The Journal of biological chemistry 2019 Sep 27;294(39):14257-14266
Neuronal Soma-Derived Degradative Lysosomes Are Continuously Delivered to Distal Axons to Maintain Local Degradation Capacity.
Farfel-Becker T, Roney JC, Cheng XT, Li S, Cuddy SR, Sheng ZH
Cell reports 2019 Jul 2;28(1):51-64.e4
Cell reports 2019 Jul 2;28(1):51-64.e4
BORC/kinesin-1 ensemble drives polarized transport of lysosomes into the axon.
Farías GG, Guardia CM, De Pace R, Britt DJ, Bonifacino JS
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 4;114(14):E2955-E2964
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 4;114(14):E2955-E2964
The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.
Schiefermeier N, Scheffler JM, de Araujo ME, Stasyk T, Yordanov T, Ebner HL, Offterdinger M, Munck S, Hess MW, Wickström SA, Lange A, Wunderlich W, Fässler R, Teis D, Huber LA
The Journal of cell biology 2014 May 26;205(4):525-40
The Journal of cell biology 2014 May 26;205(4):525-40
Salmonella exploits Arl8B-directed kinesin activity to promote endosome tubulation and cell-to-cell transfer.
Kaniuk NA, Canadien V, Bagshaw RD, Bakowski M, Braun V, Landekic M, Mitra S, Huang J, Heo WD, Meyer T, Pelletier L, Andrews-Polymenis H, McClelland M, Pawson T, Grinstein S, Brumell JH
Cellular microbiology 2011 Nov;13(11):1812-23
Cellular microbiology 2011 Nov;13(11):1812-23
Lysosomal positioning coordinates cellular nutrient responses.
Korolchuk VI, Saiki S, Lichtenberg M, Siddiqi FH, Roberts EA, Imarisio S, Jahreiss L, Sarkar S, Futter M, Menzies FM, O'Kane CJ, Deretic V, Rubinsztein DC
Nature cell biology 2011 Apr;13(4):453-60
Nature cell biology 2011 Apr;13(4):453-60
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13049-1-AP( ARL8B Antibody) at dilution of 1:1000
- Sample type
- cell line
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP( ARL8B Antibody) at dilution of 1:600
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ARL8B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ARL8B. This antibody recognizes human,mouse,rat antigen. The ARL8B antibody has been validated for the following applications: ELISA, IHC, WB analysis.