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- Antibody Data
- Antigen structure
- References [35]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- 13049-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13049-1-AP, RRID:AB_2059000
- Product name
- ARL8B antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ARL8B antibody (Cat. #13049-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55B.
Progranulin-driven lysosomal acidification facilitates exocytosis of PHEV-hijacked lysosomes for viral release.
Treatment of age-related decreases in GTP levels restores endocytosis and autophagy.
Activation of Lysosomal Retrograde Transport Triggers TPC1-IP3R1 Ca(2+) Crosstalk at Lysosome-ER MCSs Leading to Lethal Depleting of ER Calcium.
Depletion of mmu-miR-185 enhances osteocyte connectivity and suppresses bone fragility through the interaction between ARL8B and RAB5A.
Treatment of age-related decreases in GTP levels restores endocytosis and autophagy.
Lysosomal NKG7 restrains mTORC1 activity to promote CD8(+) T cell durability and tumor control.
RNF13 mediates pH- and Ca(2+)-dependent regulation of lysosomal positioning.
VPS41 recruits biosynthetic LAMP-positive vesicles through interaction with Arl8b.
ARL8B regulates lysosomal function and predicts poor prognosis in hepatocellular carcinoma.
Biogenesis of specialized lysosomes in differentiated keratinocytes relies on close apposition with the Golgi apparatus.
Macrophage-derived MMP12 promotes fibrosis through sustained damage to endothelial cells.
DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy.
Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons.
ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization.
Inhibition of endolysosome fusion increases exosome secretion.
Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes.
A program of successive gene expression in mouse one-cell embryos.
Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation.
RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.
Parkinson disease-associated Leucine-rich repeat kinase regulates UNC-104-dependent axonal transport of Arl8-positive vesicles in Drosophila.
The Salmonella effector SifA initiates a kinesin-1 and kinesin-3 recruitment process mirroring that mediated by Arl8a and Arl8b.
Specific KIF1A-adaptor interactions control selective cargo recognition.
mTORC1 activity is supported by spatial association with focal adhesions.
Lipid-mediated motor-adaptor sequestration impairs axonal lysosome delivery leading to autophagic stress and dystrophy in Niemann-Pick type C.
ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1.
Endosomal sorting drives the formation of axonal prion protein endoggresomes.
The GTPase Arl8B Plays a Principle Role in the Positioning of Interstitial Axon Branches by Spatially Controlling Autophagosome and Lysosome Location.
The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.
Hepatitis C virus infection increases autophagosome stability by suppressing lysosomal fusion through an Arl8b-dependent mechanism.
Neuronal Soma-Derived Degradative Lysosomes Are Continuously Delivered to Distal Axons to Maintain Local Degradation Capacity.
BORC/kinesin-1 ensemble drives polarized transport of lysosomes into the axon.
The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.
Salmonella exploits Arl8B-directed kinesin activity to promote endosome tubulation and cell-to-cell transfer.
Lysosomal positioning coordinates cellular nutrient responses.
Duhay V, Tian M, Kosieradzka K, Ebner M, Lo WT, Krauss M, Sprengel HL, Voss M, Riechmann M, Savas JN, Schwake M, Haucke V, Damme M
Nature communications 2026 Mar 14;17(1)
Nature communications 2026 Mar 14;17(1)
Progranulin-driven lysosomal acidification facilitates exocytosis of PHEV-hijacked lysosomes for viral release.
Wang Z, Chen Y, He W, Liu Y, Jiao Y, Wang G, Guan J, Zhao K, Zhang Q, Gao F, Li Z, Lan Y
mBio 2026 Jan 14;17(1):e0290325
mBio 2026 Jan 14;17(1):e0290325
Treatment of age-related decreases in GTP levels restores endocytosis and autophagy.
Santana RA, McWhirt JM, Brewer GJ
GeroScience 2026 Apr;48(2):2827-2850
GeroScience 2026 Apr;48(2):2827-2850
Activation of Lysosomal Retrograde Transport Triggers TPC1-IP3R1 Ca(2+) Crosstalk at Lysosome-ER MCSs Leading to Lethal Depleting of ER Calcium.
Zhu MY, Guo YJ, Zhu YQ, Wang HZ, Wang HD, Chen HY, Jiang YX, Li H, Hui H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Oct;12(39):e15313
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Oct;12(39):e15313
Depletion of mmu-miR-185 enhances osteocyte connectivity and suppresses bone fragility through the interaction between ARL8B and RAB5A.
Cui Y, Hai B, Han X, Leng H, He D, Lu Y, Wang H, Xu J, Zhao H
Stem cell research & therapy 2025 Oct 29;16(1):591
Stem cell research & therapy 2025 Oct 29;16(1):591
Treatment of age-related decreases in GTP levels restores endocytosis and autophagy.
Santana RA, McWhirt JM, Brewer GJ
bioRxiv : the preprint server for biology 2025 Jun 12;
bioRxiv : the preprint server for biology 2025 Jun 12;
Lysosomal NKG7 restrains mTORC1 activity to promote CD8(+) T cell durability and tumor control.
Ham H, Hirdler JB, Bihnam DT, Mao Z, Gicobi JK, Macedo BG, Rodriguez-Quevedo MF, Schultz DF, Correia C, Zhong J, Martinez KE, Banuelos A, Ashton DS, Lagnado AB, Guo R, Pessoa R, Pandey A, Li H, Lucien F, Borges da Silva H, Dong H, Billadeau DD
Nature communications 2025 Feb 14;16(1):1628
Nature communications 2025 Feb 14;16(1):1628
RNF13 mediates pH- and Ca(2+)-dependent regulation of lysosomal positioning.
Trinh LT, Van Vu A, Shin S, Lee C, Park SH, Cho EB, Heo Y, Kim HJ, Kim S, Yoon JB
Cell reports 2025 Aug 26;44(8):116053
Cell reports 2025 Aug 26;44(8):116053
VPS41 recruits biosynthetic LAMP-positive vesicles through interaction with Arl8b.
Sanzà P, van der Beek J, Draper D, de Heus C, Veenendaal T, Brink CT, Farías GG, Liv N, Klumperman J
The Journal of cell biology 2025 Apr 3;224(4)
The Journal of cell biology 2025 Apr 3;224(4)
ARL8B regulates lysosomal function and predicts poor prognosis in hepatocellular carcinoma.
Wu L, Weng Z, Yang X, Huang Y, Lin Y, Li S, Fu L, Yun J
Scientific reports 2025 Apr 10;15(1):12278
Scientific reports 2025 Apr 10;15(1):12278
Biogenesis of specialized lysosomes in differentiated keratinocytes relies on close apposition with the Golgi apparatus.
Mahanty S, Bergam P, Belapurkar V, Eluvathingal L, Gupta N, Goud B, Nair D, Raposo G, Setty SRG
Cell death & disease 2024 Jul 11;15(7):496
Cell death & disease 2024 Jul 11;15(7):496
Macrophage-derived MMP12 promotes fibrosis through sustained damage to endothelial cells.
Zhou X, Zhang C, Yang S, Yang L, Luo W, Zhang W, Zhang X, Chao J
Journal of hazardous materials 2024 Jan 5;461:132733
Journal of hazardous materials 2024 Jan 5;461:132733
DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy.
Kumar R, Khan M, Francis V, Aguila A, Kulasekaran G, Banks E, McPherson PS
Nature communications 2024 Jan 31;15(1):919
Nature communications 2024 Jan 31;15(1):919
Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons.
Li CH, Kersten N, Özkan N, Nguyen DTM, Koppers M, Post H, Altelaar M, Farias GG
Nature communications 2024 Dec 30;15(1):10829
Nature communications 2024 Dec 30;15(1):10829
ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization.
Menon D, Bhapkar A, Manchandia B, Charak G, Rathore S, Jha RM, Nahak A, Mondal M, Omrane M, Bhaskar AK, Thukral L, Thiam AR, Gandotra S
Cell reports 2023 Oct 31;42(10):113203
Cell reports 2023 Oct 31;42(10):113203
Inhibition of endolysosome fusion increases exosome secretion.
Shelke GV, Williamson CD, Jarnik M, Bonifacino JS
The Journal of cell biology 2023 Jun 5;222(6)
The Journal of cell biology 2023 Jun 5;222(6)
Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes.
Schleinitz A, Pöttgen LA, Keren-Kaplan T, Pu J, Saftig P, Bonifacino JS, Haas A, Jeschke A
Cell reports 2023 Jan 31;42(1):111969
Cell reports 2023 Jan 31;42(1):111969
A program of successive gene expression in mouse one-cell embryos.
Asami M, Lam BYH, Hoffmann M, Suzuki T, Lu X, Yoshida N, Ma MK, Rainbow K, Gužvić M, VerMilyea MD, Yeo GSH, Klein CA, Perry ACF
Cell reports 2023 Feb 28;42(2):112023
Cell reports 2023 Feb 28;42(2):112023
Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation.
Bauer CS, Webster CP, Shaw AC, Kok JR, Castelli LM, Lin YH, Smith EF, Illanes-Álvarez F, Higginbottom A, Shaw PJ, Azzouz M, Ferraiuolo L, Hautbergue GM, Grierson AJ, De Vos KJ
Frontiers in cellular neuroscience 2022;16:1061559
Frontiers in cellular neuroscience 2022;16:1061559
RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.
Keren-Kaplan T, Sarić A, Ghosh S, Williamson CD, Jia R, Li Y, Bonifacino JS
Nature communications 2022 Mar 21;13(1):1506
Nature communications 2022 Mar 21;13(1):1506
Parkinson disease-associated Leucine-rich repeat kinase regulates UNC-104-dependent axonal transport of Arl8-positive vesicles in Drosophila.
Inoshita T, Liu JY, Taniguchi D, Ishii R, Shiba-Fukushima K, Hattori N, Imai Y
iScience 2022 Dec 22;25(12):105476
iScience 2022 Dec 22;25(12):105476
The Salmonella effector SifA initiates a kinesin-1 and kinesin-3 recruitment process mirroring that mediated by Arl8a and Arl8b.
Fang Z, Fallet M, Moest T, Gorvel JP, Méresse S
Journal of cell science 2022 Dec 1;135(1)
Journal of cell science 2022 Dec 1;135(1)
Specific KIF1A-adaptor interactions control selective cargo recognition.
Hummel JJA, Hoogenraad CC
The Journal of cell biology 2021 Oct 4;220(10)
The Journal of cell biology 2021 Oct 4;220(10)
mTORC1 activity is supported by spatial association with focal adhesions.
Rabanal-Ruiz Y, Byron A, Wirth A, Madsen R, Sedlackova L, Hewitt G, Nelson G, Stingele J, Wills JC, Zhang T, Zeug A, Fässler R, Vanhaesebroeck B, Maddocks ODK, Ponimaskin E, Carroll B, Korolchuk VI
The Journal of cell biology 2021 May 3;220(5)
The Journal of cell biology 2021 May 3;220(5)
Lipid-mediated motor-adaptor sequestration impairs axonal lysosome delivery leading to autophagic stress and dystrophy in Niemann-Pick type C.
Roney JC, Li S, Farfel-Becker T, Huang N, Sun T, Xie Y, Cheng XT, Lin MY, Platt FM, Sheng ZH
Developmental cell 2021 May 17;56(10):1452-1468.e8
Developmental cell 2021 May 17;56(10):1452-1468.e8
ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1.
Keren-Kaplan T, Bonifacino JS
Current biology : CB 2021 Feb 8;31(3):540-554.e5
Current biology : CB 2021 Feb 8;31(3):540-554.e5
Endosomal sorting drives the formation of axonal prion protein endoggresomes.
Chassefeyre R, Chaiamarit T, Verhelle A, Novak SW, Andrade LR, Leitão ADG, Manor U, Encalada SE
Science advances 2021 Dec 24;7(52):eabg3693
Science advances 2021 Dec 24;7(52):eabg3693
The GTPase Arl8B Plays a Principle Role in the Positioning of Interstitial Axon Branches by Spatially Controlling Autophagosome and Lysosome Location.
Adnan G, Rubikaite A, Khan M, Reber M, Suetterlin P, Hindges R, Drescher U
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Oct 14;40(42):8103-8118
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Oct 14;40(42):8103-8118
The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.
Lu M, van Tartwijk FW, Lin JQ, Nijenhuis W, Parutto P, Fantham M, Christensen CN, Avezov E, Holt CE, Tunnacliffe A, Holcman D, Kapitein L, Schierle GSK, Kaminski CF
Science advances 2020 Dec;6(51)
Science advances 2020 Dec;6(51)
Hepatitis C virus infection increases autophagosome stability by suppressing lysosomal fusion through an Arl8b-dependent mechanism.
Jones-Jamtgaard KN, Wozniak AL, Koga H, Ralston R, Weinman SA
The Journal of biological chemistry 2019 Sep 27;294(39):14257-14266
The Journal of biological chemistry 2019 Sep 27;294(39):14257-14266
Neuronal Soma-Derived Degradative Lysosomes Are Continuously Delivered to Distal Axons to Maintain Local Degradation Capacity.
Farfel-Becker T, Roney JC, Cheng XT, Li S, Cuddy SR, Sheng ZH
Cell reports 2019 Jul 2;28(1):51-64.e4
Cell reports 2019 Jul 2;28(1):51-64.e4
BORC/kinesin-1 ensemble drives polarized transport of lysosomes into the axon.
Farías GG, Guardia CM, De Pace R, Britt DJ, Bonifacino JS
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 4;114(14):E2955-E2964
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 4;114(14):E2955-E2964
The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.
Schiefermeier N, Scheffler JM, de Araujo ME, Stasyk T, Yordanov T, Ebner HL, Offterdinger M, Munck S, Hess MW, Wickström SA, Lange A, Wunderlich W, Fässler R, Teis D, Huber LA
The Journal of cell biology 2014 May 26;205(4):525-40
The Journal of cell biology 2014 May 26;205(4):525-40
Salmonella exploits Arl8B-directed kinesin activity to promote endosome tubulation and cell-to-cell transfer.
Kaniuk NA, Canadien V, Bagshaw RD, Bakowski M, Braun V, Landekic M, Mitra S, Huang J, Heo WD, Meyer T, Pelletier L, Andrews-Polymenis H, McClelland M, Pawson T, Grinstein S, Brumell JH
Cellular microbiology 2011 Nov;13(11):1812-23
Cellular microbiology 2011 Nov;13(11):1812-23
Lysosomal positioning coordinates cellular nutrient responses.
Korolchuk VI, Saiki S, Lichtenberg M, Siddiqi FH, Roberts EA, Imarisio S, Jahreiss L, Sarkar S, Futter M, Menzies FM, O'Kane CJ, Deretic V, Rubinsztein DC
Nature cell biology 2011 Apr;13(4):453-60
Nature cell biology 2011 Apr;13(4):453-60
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13049-1-AP( ARL8B Antibody) at dilution of 1:1000
- Sample type
- cell line
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP( ARL8B Antibody) at dilution of 1:600
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ARL8B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ARL8B. This antibody recognizes human,mouse,rat antigen. The ARL8B antibody has been validated for the following applications: ELISA, IHC, WB analysis.
