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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- ELISA [1]
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- Product number
- PAB14092 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14092, RRID:AB_10676392
- Product name
- SOX4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SOX4.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The SRY-HMG box gene, SOX4, is a target of gene amplification at chromosome 6p in lung cancer.
Medina PP, Castillo SD, Blanco S, Sanz-Garcia M, Largo C, Alvarez S, Yokota J, Gonzalez-Neira A, Benitez J, Clevers HC, Cigudosa JC, Lazo PA, Sanchez-Cespedes M
Human molecular genetics 2009 Apr 1;18(7):1343-52
Human molecular genetics 2009 Apr 1;18(7):1343-52
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with SOX4 polyclonal antibody (Cat # PAB14092) as described above.The H522 cells are known to overexpress SOX4.Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- H1299 (lung carcinoma cells) were transfected with HA-tagged SOX4 (Lane 1) or with a HA-tagged truncated SOX4 (395X mutant, Lane 2).Whole cell extracts (60~100 ug) were analysed by Western blot using SOX4 polyclonal antibody (Cat # PAB14092) diluted 1 : 4,000 in TBS-Tween containing 2% skimmed milk.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of SOX4 polyclonal antibody (Cat # PAB14092). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 18,000.
- Validation comment
- ELISA
