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- Immunocytochemistry [1]
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- Product number
- PA5-72852 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Circ-DONSON Knockdown Inhibits Cell Proliferation and Radioresistance of Breast Cancer Cells via Regulating SOX4.
Zhu X, Li L
Journal of oncology 2021;2021:8461740
Journal of oncology 2021;2021:8461740
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SOX4 in rat testis cells. The samples were probed with a SOX4 polyclonal antibody (Product # PA5-72852) at a dilution of 20 µg/ml.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of SOX4 in rat testis tissue with SOX4 Polyclonal Antibody (Product # PA5-72852) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of SOX4 in rat testis tissue with SOX4 Polyclonal Antibody (Product # PA5-72852) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Circ-DONSON knockdown inhibited BC cell proliferation, reduced the radioresistance of BC cells, and inhibited SOX4 expression. (a) The expression of circ-DONSON in MCF-7 and MDA-MB-231 cells after treatment with circ-DONSON siRNAs. (b) CCK-8 assay was performed on MCF-7 and MDA-MB-231 cells after transfection with circ-DONSON siRNAs. (c) BrdU-ELISA assay was conducted on MCF-7 and MDA-MB-231 cells after transfection with circ-DONSON siRNAs. (d) Cell survival assay was performed on MCF-7 and MDA-MB-231 cells after exposure to 0, 2, 4, 6, or 8 Gy X-ray irradiation and transfection of circ-DONSON siRNAs. (e) Cell viability of MCF-7 and MDA-MB-231 cells after exposure to 4 Gy X-ray irradiation and transfection of circ-DONSON siRNAs was analyzed by CCK-8 assay. (f) The relative caspase-3 viability of MCF-7 and MDA-MB-231 cells after exposure to 4 Gy X-ray irradiation and treatment of circ-DONSON siRNAs. (g) The protein levels of SOX4 in MCF-7 and MDA-MB-231 cells after treatment with circ-DONSON siRNAs. The density of western blots bands was analyzed by ImageJ software. * P < 0.05, ** P < 0.01, and *** P < 0.001.
