Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-72163 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOX4 in A2058 cell lysate using 35 µg of protein per lane. Samples were incubated with SOX4 polyclonal antibody (Product # PA5-72163).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SOX4 in HeLa cells. Samples were fixed with 4% paraformaldehyde (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/mL, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SOX4 in HeLa cells. Samples were fixed with 4% paraformaldehyde (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/mL, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SOX4 in paraffin-embedded human brain tissue. Samples were incubated with SOX4 polyclonal antibody (Product # PA5-72163) followed by a peroxidase-conjugate secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of SOX4 in A2058 cells (right histogram) compared to a negative control cell (left histogram). Samples were incubated with SOX4 polyclonal antibody (Product # PA5-72163) followed by a FITC conjugated goat anti-rabbit secondary antibody.