Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-63768 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYLK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IEFQAVPSEK SEVGQALCLT AREEDCFQIL DDCPPPPAPF PHRMVELRTG NVSSEFSMNS KEALGGGKFG AVCTCMEKAT Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 89%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYLK2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a MYLK2 Polyclonal Antibody (Product # PA5-63768).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MYLK2 in human cell line U-2 OS using a MYLK2 Polyclonal Antibody (Product # PA5-63768) shows localization to endoplasmic reticulum.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MYLK2 in human skeletal muscle shows moderate cytoplasmic positivity in myocytes. Samples were probed using a MYLK2 Polyclonal Antibody (Product # PA5-63768).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MYLK2 in human skeletal muscle and heart muscle tissues using MYLK2 Polyclonal Antibody (Product # PA5-63768). Corresponding MYLK2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MYLK2 in human heart muscle using MYLK2 Polyclonal Antibody (Product # PA5-63768) shows low expression as expected.