Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
- Other assay [1]
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Validation data
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- Product number
- 17947-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RGN/SMP30 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSSIKIECV LPENCRCGES PVWEEVSNSL LFVDIPAKKV CRWDSFTKQV QRVTMDAPVS SVALRQSGGY VATIGTKFCA LNWKEQSAVV LATVDNDKKN NRFNDGKVDP AGRYFAGTMA EETAPAVLER HQGALYSLFP DHHVKKYFDQ VDISNGLDWS LDHKIFYYID SLSYSVDAFD YDLQTGQISN RRSVYKLEKE EQIPDGMCID AEGKLWVACY NGGRVIRLDP VTGKRLQTVK LPVDKTTSCC FGGKNYSEMY VTCARDGMDP EGLLRQPEAG GIFKITGLGV KGIAPYSYAG (1-299 aa encoded by BC073173)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using (Product # 17947-1-AP) (RGN/SMP30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver slide using 17947-1-AP ( RGN/SMP30 antibody at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver slide using 17947-1-AP ( RGN/SMP30 antibody at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RGN/SMP30 (IP:17947-1-AP, 4ug; Detection:17947-1-AP 1:1000) with mouse liver tissue lysate 4000 ug.