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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
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- Product number
- GTX129443 - Provider product page
- Provider
- GeneTex
- Product name
- ARID2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- ARID2 antibody detects ARID2 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extractB. 30 £gg whole cell lysate/extract of human ARID2-transfected 293T cells5 % SDS-PAGEARID2 antibody (GTX129443) dilution: 1:5000
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- GeneTex (provider)
- Main image
- Experimental details
- ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at a dilution of 1:3000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at 1:3000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARID2 antibody detects ARID2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ARID2 protein stained by ARID2 antibody (GTX129443) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARID2 antibody immunoprecipitates ARID2 protein in IP experiments.IP samples: HeLa whole cell extractA. 35 £gg HeLa whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of ARID2 protein by 4 £gg ARID2 antibody (GTX129443)D. Immunoprecipitation of ARID2 protein by 4 £gg ARID2 antibody (GTX129444) 5 % SDS-PAGEThe immunoprecipitated ARID2 protein was detected by ARID2 antibody (GTX129443) diluted at 1:3000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ARID2 protein from HepG2 whole cell extracts using 5 £gg of ARID2 antibody (GTX129443).Western blot analysis was performed using ARID2 antibody (GTX129443).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
