GTX129443

ARID2 antibody from GeneTex

BAF200, DKFZp686G052, FLJ30619, KIAA1557

Western blot

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Immunocytochemistry

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Immunoprecipitation

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Provider product page for GTX129443

Antibody Data

Product number

GTX129443

Provider

GeneTex

Product name

ARID2 antibody

Provider product page

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ARID2 antibody detects ARID2 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extractB. 30 £gg whole cell lysate/extract of human ARID2-transfected 293T cells5 % SDS-PAGEARID2 antibody (GTX129443) dilution: 1:5000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at a dilution of 1:3000.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at 1:3000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ARID2 antibody detects ARID2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ARID2 protein stained by ARID2 antibody (GTX129443) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ARID2 antibody immunoprecipitates ARID2 protein in IP experiments.IP samples: HeLa whole cell extractA. 35 £gg HeLa whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of ARID2 protein by 4 £gg ARID2 antibody (GTX129443)D. Immunoprecipitation of ARID2 protein by 4 £gg ARID2 antibody (GTX129444) 5 % SDS-PAGEThe immunoprecipitated ARID2 protein was detected by ARID2 antibody (GTX129443) diluted at 1:3000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of ARID2 protein from HepG2 whole cell extracts using 5 £gg of ARID2 antibody (GTX129443).Western blot analysis was performed using ARID2 antibody (GTX129443).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.