PA5-35857
antibody from Invitrogen Antibodies
Targeting: ARID2
BAF200, DKFZp686G052, FLJ30619, KIAA1557
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-35857 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARID2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, human ARID2-transfected 293T.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID2 using Various whole cell extracts (30 µg). Samples were loaded onto a 5% SDS-PAGE gel and probed with an ARID2 polyclonal antibody (Product # PA5-35857) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 Polyclonal Antibody (Product # PA5-35857) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody detects ARID2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human ARID2-transfected 293T cells.5 % SDS-PAGE. ARID2 Polyclonal Antibody (Product # PA5-35857) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody detects ARID2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ARID2 protein stained by ARID2 Polyclonal Antibody (Product # PA5-35857) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.