PA5-85572
antibody from Invitrogen Antibodies
Targeting: ARID2
BAF200, DKFZp686G052, FLJ30619, KIAA1557
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-85572 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARID2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID2 in A) 293T cells, B) of human ARID2-transfected 293T cells using ARID2 polyclonal antibody (Product # PA5-85572) using 30 µg of sample at a dilution of 1:5000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID2 in whole cell extract using ARID2 polyclonal antibody (Product # PA5-85572) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID2 in various whole cell extracts using ARID2 polyclonal antibody (Product # PA5-85572) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID2 in A) HeLa whole cell lysate, B) HeLa nuclear lysate using ARID2 polyclonal antibody (Product # PA5-85572) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 Polyclonal Antibody (Product # PA5-85572) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody detects ARID2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 Polyclonal Antibody (Product # PA5-85572) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody detects ARID2 protein by western blot analysis. A. 30 µg 293T. B. 30 µg of human ARID2-transfected 293T cells.5% SDS-PAGE. ARID2 Polyclonal Antibody (Product # PA5-85572) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody detects ARID2 protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg HeLa nuclear lysate/extract.5% SDS-PAGE. ARID2 Polyclonal Antibody (Product # PA5-85572) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody detects ARID2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ARID2 protein stained by ARID2 Polyclonal Antibody (Product # PA5-85572) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody detects ARID2 protein on human hepatoma and human normal liver by immunohistochemical analysis. Sample: (a) Paraffin-embedded human hepatoma (b) Paraffin-embedded human normal liver. ARID2 Polyclonal Antibody (Product # PA5-85572) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ARID2 in HepG2 whole cell extract, A) control with preimmune rabbit IgG, B) immunoprecipitation of ATG9A protein with ARID2 polyclonal antibody (Product # PA5-85572) using 4 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID2 Polyclonal Antibody immunoprecipitates ARID2 protein in IP experiments. IP samples: HepG2 whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of ARID2 protein by 4 µg ARID2 Polyclonal Antibody (Product # PA5-85572). 5 % SDS-PAGE. The immunoprecipitated ARID2 protein was detected by ARID2 Polyclonal Antibody (Product # PA5-85572) diluted at 1:500.