A302-230A

antibody from Invitrogen Antibodies

Targeting: ARID2 BAF200, DKFZp686G052, FLJ30619, KIAA1557

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-230A

Provider

Invitrogen Antibodies

Product name

ARID2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ARID2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody (Product # A302-230A; lot A302-230A-2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ARID2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARID2 antibody A302-230A used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. ARID2 was also immunoprecipitated by rabbit anti-ARID2 antibody A302-229A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ARID2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID2 (Product # A302-230A; lot 2) used at a dilution of 1:5,000 ( 0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ARID2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ARID2 (Product # A302-230A lot 2) used at a dilution of 1:5,000 ( 0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ARID2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARID2 (Cat. No. A302-230A) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ARID2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID2 antibody (Product # A302-230A; lot A302-230A-2) used for IP at 6 µg per reaction. ARID2 was also immunoprecipitated by a previous lot of this antibody (lot A302-230A-1). For blotting immunoprecipitated ARID2 (Product # A302-230A) was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.