NBP1-26615
antibody from Novus Biologicals
Targeting: ARID2
BAF200, DKFZp686G052, FLJ30619, KIAA1557
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-26615 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-26615, RRID:AB_1852787
- Product name
- Rabbit Polyclonal ARID2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARID2 Antibody [NBP1-26615] - Whole cell lysate from HeLa cells. ARID2 was also immunoprecipitated by rabbit anti-ARID2 antibody NBP1-26614.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARID2 Antibody [NBP1-26615] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ARID2 Antibody [NBP1-26615] - Mouse embryonic fibroblasts. Verified image from customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ARID2 Antibody [NBP1-26615] - Detection of human ARID2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID2 antibody NBP1-26615 (lot NBP1-26615-2) used for IP at 6 ug per reaction. ARID2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-26615-1). For blotting immunoprecipitated ARID2, NBP1-26615 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID2 used at a dilution of 1:5,000 ( 0.2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ARID2 used at a dilution of 1:5,000 ( 0.2ug/ml). Detection: DAB