MA5-27593

antibody from Invitrogen Antibodies

Targeting: NALCN bA430M15.1, CanIon, VGCNL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27593

Provider

Invitrogen Antibodies

Product name

NALCN Monoclonal Antibody (S187-7)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S187-7

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NALCN in rat brain with 15 µg of sample. The sample was incubated with NALCN monoclonal antibody (Product # MA5-27593) using a dilution of 1:1000 (16 hours at 4°C), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight (MW) Ladder, Lane 3: Rat Brain membrane.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NALCN in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with NALCN monoclonal antibody (Product # MA5-27593) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) NALCN Antibody, D) Merged image. Magnification: 60x.