ASC-022-50UL

antibody from Invitrogen Antibodies

Targeting: NALCN bA430M15.1, CanIon, VGCNL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ASC-022-50UL

Provider

Invitrogen Antibodies

Product name

NALCN/VGCNL1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and human SH-SYS5 brain neuroblastoma cell (lanes 3 and 6) lysates: - 1-3. Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:200).4-6. Anti-NALCN/VGCNL1 (extracellular) Antibody , preincubated with NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and human SH-SYS5 brain neuroblastoma cell (lanes 3 and 6) lysates: - 1-3. Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:200).4-6. Anti-NALCN/VGCNL1 (extracellular) Antibody , preincubated with NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of NALCN in rat PC12 cells - Cell surface detection of NALCN in intact living rat pheochromocytoma PC12 cells. Extracellular staining of cells using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:50) followed by goat Anti-rabbit-AlexaFluor-594secondary Antibody . Merged image of extracellular staining with live image of the cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of NALCN in rat PC12 cells - Cell surface detection of NALCN in intact living rat pheochromocytoma PC12 cells. Extracellular staining of cells using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:50) followed by goat Anti-rabbit-AlexaFluor-594secondary Antibody . Merged image of extracellular staining with live image of the cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of NALCN in rat hypothalamus - Immunohistochemical staining of rat hypothalamus using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022). A. NALCN (green) staining appears in the supra-optic nucleus (SON). B. DAPI is used as the counterstain (blue). White arrows point at the sharp border between the SON and optic tract (OT). C. Merged images of A and B.