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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
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- Product number
- H00004745-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004745-B01P, RRID:AB_1577661
- Product name
- NELL1 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human NELL1 protein.
- Antigen sequence
MPMDLILVVWFCVCTARTVVGFGMDPDLQMDIVTE
LDLVNTTLGVAQVSGMHNASKAFLFQDIEREIHAA
PHVSEKLIQLFRNKSEFTILATVQQKPSTSGVILS
IRELEHSYFELESSGLRDEIRYHYIHNGKPRTEAL
PYRMADGQWHKVALSVSASHLLLHVDCNRIYERVI
DPPDTNLPPGINLWLGQRNQKHGLFKGIIQDGKII
FMPNGYITQCPNLNHTCPTCSDFLSLVQGIMDLQE
LLAKMTAKLNYAETRLSQLENCHCEKTCQVSGLLY
RDQDSWVDGDHCRNCTCKSGAVECRRMSCPPLNCS
PDSLPVHIAGQCCKVCRPKCIYGGKVLAEGQRILT
KSCRECRGGVLVKITEMCPPLNCSEKDHILPENQC
CRVCRGHNFCAEGPKCGENSECKNWNTKATCECKS
GYISVQGDSAYCEDIDECAAKMHYCHANTVCVNLP
GLYRCDCVPGYIRVDDFSCTEHDECGSGQHNCDEN
AICTNTVQGHSCTCKPGYVGNGTICRAFCEEGCRY
GGTCVAPNKCVCPSGFTGSHCEKDIDECSEGIIEC
HNHSRCVNLPGWYHCECRSGFHDDGTYSLSGESCI
DIDECALRTHTCWNDSACINLAGGFDCLCPSGPSC
SGDCPHEGGLKHNGQVWTLKEDRCSVCSCKDGKIF
CRRTACDCQNPSADLFCCPECDTRVTSQCLDQNGH
KLYRSGDNWTHSCQQCRCLEGEVDCWPLTCPNLSC
EYTAILEGECCPRCVSDPCLADNITYDIRKTCLDS
YGVSRLSGSVWTMAGSPCTTCKCKNGRVCCSVDFE
CLQNN- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Oligomerization-induced conformational change in the C-terminal region of Nel-like molecule 1 (NELL1) protein is necessary for the efficient mediation of murine MC3T3-E1 cell adhesion and spreading.
Efficient production and characterization of recombinant human NELL1 protein in human embryonic kidney 293-F cells.
Nakamura Y, Hasebe A, Takahashi K, Iijima M, Yoshimoto N, Maturana AD, Ting K, Kuroda S, Niimi T
The Journal of biological chemistry 2014 Apr 4;289(14):9781-94
The Journal of biological chemistry 2014 Apr 4;289(14):9781-94
Efficient production and characterization of recombinant human NELL1 protein in human embryonic kidney 293-F cells.
Hasebe A, Tashima H, Ide T, Iijima M, Yoshimoto N, Ting K, Kuroda S, Niimi T
Molecular biotechnology 2012 May;51(1):58-66
Molecular biotechnology 2012 May;51(1):58-66
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NELL1 recombinant protein (H00004745-P01) by NELL1 purified MaxPab mouse polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NELL1 expression in transfected 293T cell line (H00004745-T01) by NELL1 MaxPab polyclonal antibody.Lane 1: NELL1 transfected lysate(89.1 KDa).Lane 2: Non-transfected lysate.
