Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF7568 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal PD-ECGF/Thymidine Phosphorylase Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects PD-ECGF/Thymidine Phosphorylase in direct ELISAs. Detects human, mouse, and rat PD-ECGF/Thymidine Phosphorylase in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Preclinical investigation of potential use of thymidine phosphorylase-targeting tracer for diagnosis of nonalcoholic steatohepatitis.
Higashikawa K, Horiguchi S, Tarisawa M, Shibata Y, Ohkura K, Yasui H, Takeda H, Kuge Y
Nuclear medicine and biology 2020 Mar - Apr;82-83:25-32
Nuclear medicine and biology 2020 Mar - Apr;82-83:25-32
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse PD-ECGF/Thymidine Phosphorylase by Western Blot. Western blot shows lysates of L1210 mouse lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat PD-ECGF/Thymidine Phosphorylase by Western Blot. Detection of Human, Mouse, and Rat PD-ECGF/Thymidine Phosphorylase by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- PD-ECGF/Thymidine Phosphorylase in Mouse Embryonic Brain. PD-ECGF/Thymidine Phosphorylase in Mouse Embryonic Brain.
