Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB7568-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse PD-ECGF/Thymidine Phosphorylase in direct ELISAs. Detects human, mouse, and rat PD-ECGF/Thymidine Phosphorylase in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q99N42- Isotype
- IgG
- Antibody clone number
- 826722
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse PD-ECGF/Thymidine Phosphorylase by Western Blot. Western blot shows lysates of Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse PD-ECGF/Thymidine Phosphorylase Monoclonal Antibody (Catalog # MAB7568) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PD-ECGF/ Thymidine Phosphorylase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PD-ECGF/Thymidine Phosphorylase in Mouse Embryo. PD-ECGF/Thymidine Phosphorylase was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse PD-ECGF/Thymidine Phosphorylase Monoclonal Antibody (Catalog # MAB7568) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
