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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-28611 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LARGE Monoclonal Antibody (LARGE-02)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase localizing mainly to the Golgi apparatus. Crossreactivity with LARGE2 was not determined.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LARGE-02
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LARGE in HEK293 cells. Sample was incubated with LARGE monoclonal antibody (Product # MA5-28611). Results are displayed as follows: A) transfected cells, B) non-transfected cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separation of Hek293 cells transfected with LARGE1 vector and stained using anti-LARGE1 (LARGE-02) purified Monoclonal antibody (Product # MA5-28611), concentration in sample 15 µg/mL (GAM APC, red-filled) from Hek293 cells transfected with LARGE1 and unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
