12434-1-AP

antibody from Invitrogen Antibodies

Targeting: STAM STAM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12434-1-AP

Provider

Invitrogen Antibodies

Product name

STAM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VNHKDPHVA MQALTLLGAC VSNCGKIFHL EVCSRDFASE VSNVLNKGHP KVCEKLKALM VEWTDEFKND PQLSLISAMI KNLKEQGVTF PAIGSQAAEQ AKASPALVAK DPGTVANKKE EEDLAKAIEL SLKEQRQQST TLSTLYPSTS SLLTNHQHEG RKVRAIYDFE AAEDNELTFK AGEIITVLDD SDPNWWKGET HQGIGLFPSN FVTAYLTAEP EMIKTEKKTV QFSDDVQVET IEPEPEPAFI DEDKMDQLLQ MLQSTDPSDD QPDLPELLHL EAMCHQMGPL IDEKLEDIDR KHSELSELNV KVMEALSLYT KLMNEDPMYS MYAKLQNQPG SGPTIRKPSP S (54-403 aa encoded by BC030586)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.4 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of 12434-1-AP (STAM antibody).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

mouse brain tissue were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 12434-1-AP (STAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 12434-1-AP ( STAM antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 12434-1-AP ( STAM antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-STAM (IP:12434-1-AP, 4ug; Detection:12434-1-AP 1:300) with PC-3 cells lysate 1440ug.