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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-50325 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STAM Recombinant Rabbit Monoclonal Antibody (HL1635)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL1635
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of STAM using STAM Recombinant Monoclonal Antibody (Product # MA5-50325) in Jurkat (30 μg) and Raji (30 μg) whole cell extracts at a dilution of 1:1,000. Extracts were separated by 10% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of STAM using STAM Recombinant Monoclonal Antibody (Product # MA5-50325) in Non-transfected (–) and transfected (+) 293T whole cell extracts at a dilution of 1:100,000. Extracts were separated by 10% SDS PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of STAM using STAM Recombinant Monoclonal Antibody (Product # MA5-50325) in HeLa cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: STAM stained by STAM antibody (MA5-50325) diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of STAM using STAM Recombinant Monoclonal Antibody (Product # MA5-50325) in Paraffin embedded mouse stomach tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.
