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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-78610 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSLP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: human kidney
- Concentration
- 1 mg/mL
Submitted references Human Lung-Resident Macrophages Express and Are Targets of Thymic Stromal Lymphopoietin in the Tumor Microenvironment.
Braile M, Fiorelli A, Sorriento D, Di Crescenzo RM, Galdiero MR, Marone G, Santini M, Varricchi G, Loffredo S
Cells 2021 Aug 6;10(8)
Cells 2021 Aug 6;10(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TSLP in human tissue extracts using 30 µg of protein. Samples were separated with 15 % SDS-PAGE and incubated with TSLP polyclonal antibody (Product # PA5-78610) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TSLP Polyclonal Antibody detects TSLP protein by western blot analysis. Human tissue extracts (30 µg) was separated by 15 % SDS-PAGE, and the membrane was blotted with TSLP Polyclonal Antibody (Product # PA5-78610) diluted by 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- An ELISA plate is coated with 50 µL of TSLP recombinant protein at concentration of 2 µg/mL. The coated protein is detected with TSLP Polyclonal Antibody (Product # PA5-78610) at concentration ranged from 31.25 to 500 ng/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TSLP Polyclonal Antibody detects TSLP protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. TSLP Polyclonal Antibody (Product # PA5-78610) diluted at 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Constitutive expression of TSLP system in human lung macrophages (HLMs). The constitutive expression of sfTSLP, lfTSLP, TSLPR and IL-7Ralpha mRNAs was evaluated by quantitative RT-PCR in highly purified HLMs (4.5 x 10 6 cells/well) ( A ). Data are mean +- SD of 6 independent experiments obtained from different patients. Cytocentrifuge preparations of HLMs were immunohistochemically stained for TSLP ( C ), TSLPR ( D ), and IL-7Ralpha ( E ) with specific primary antibodies or in absence ( B ) of primary antibody (CTRL) as described in Materials and Methods. Microscope magnification 60x. Results are representative of 6 independent experiments obtained from different patients. * p < 0.01 when compared to lfTSLP, TSLPR, IL-7Ralpha. px: pixels.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Expression of TSLP, TSLPR, and IL-7Ralpha in peritumoral and intratumoral human lung cancer by immunohistochemistry. Immunohistochemical staining for TSLP, TSLPR, and IL-7Ralpha in peritumoral and intratumoral human lung cancer. In the control (CTRL), the primary antibody was omitted. Microscope magnification 20x. This experiment is representative of 5 experiments obtained from different patients.
