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- Product number
- PA5-78610 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSLP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: human kidney Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Human Lung-Resident Macrophages Express and Are Targets of Thymic Stromal Lymphopoietin in the Tumor Microenvironment.
Braile M, Fiorelli A, Sorriento D, Di Crescenzo RM, Galdiero MR, Marone G, Santini M, Varricchi G, Loffredo S
Cells 2021 Aug 6;10(8)
Cells 2021 Aug 6;10(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- An ELISA plate is coated with 50 µL of TSLP recombinant protein at concentration of 2 µg/mL. The coated protein is detected with TSLP Polyclonal Antibody (Product # PA5-78610) at concentration ranged from 31.25 to 500 ng/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Constitutive expression of TSLP system in human lung macrophages (HLMs). The constitutive expression of sfTSLP, lfTSLP, TSLPR and IL-7Ralpha mRNAs was evaluated by quantitative RT-PCR in highly purified HLMs (4.5 x 10 6 cells/well) ( A ). Data are mean +- SD of 6 independent experiments obtained from different patients. Cytocentrifuge preparations of HLMs were immunohistochemically stained for TSLP ( C ), TSLPR ( D ), and IL-7Ralpha ( E ) with specific primary antibodies or in absence ( B ) of primary antibody (CTRL) as described in Materials and Methods. Microscope magnification 60x. Results are representative of 6 independent experiments obtained from different patients. * p < 0.01 when compared to lfTSLP, TSLPR, IL-7Ralpha. px: pixels.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Expression of TSLP, TSLPR, and IL-7Ralpha in peritumoral and intratumoral human lung cancer by immunohistochemistry. Immunohistochemical staining for TSLP, TSLPR, and IL-7Ralpha in peritumoral and intratumoral human lung cancer. In the control (CTRL), the primary antibody was omitted. Microscope magnification 20x. This experiment is representative of 5 experiments obtained from different patients.
