PA5-20320

antibody from Invitrogen Antibodies

Targeting: TSLP

Western blot

Antibody data

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Validation data

Product number: PA5-20320 - Provider product page
Provider: Invitrogen Antibodies
Product name: TSLP Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is A-20 cell lysate. PA5-20320 can be used with blocking peptide PEP-0440.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4°C

TSLP protein structure - PA5-20320 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling TSLP with TSLP Polyclonal Antibody (Product # PA5-20320) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling TSLP with TSLP Polyclonal Antibody (Product # PA5-20320) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using TSLP Polyclonal Antibody (Product # PA5-20320) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using TSLP Polyclonal Antibody (Product # PA5-20320) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.