Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- PA5-20320 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSLP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is A-20 cell lysate. PA5-20320 can be used with blocking peptide PEP-0440.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Noncanonical NF-κB signaling and the essential kinase NIK modulate crucial features associated with eosinophilic esophagitis pathogenesis.
Eden K, Rothschild DE, McDaniel DK, Heid B, Allen IC
Disease models & mechanisms 2017 Dec 19;10(12):1517-1527
Disease models & mechanisms 2017 Dec 19;10(12):1517-1527
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of A-20 cell lysate using a TSLP polyclonal antibody (Product # PA5-20320) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Heart (Lane 1) and Human Prostate (Lane 2). Loading: 15 µg of lysates per lane. Antibodies: TSLP Polyclonal Antibody (Product # PA5-20320) (4 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Mouse A-20 Cell Line. Loading: 15 µg of lysates per lane. Antibodies: TSLP Polyclonal Antibody (Product # PA5-20320) (A: 0.5 µg/mL, B: 1 µg/mL, C: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation of TSLP with Human Recombinant protein. Loading: 30 ng of TSLP partial human recombinant protein per lane. Antibodies: TSLP Polyclonal Antibody (Product # PA5-20320) (Lane 1: 0.25 µg/mL, Lane 2: 0.5 µg/mL, Lane 3: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. TLSP partial human recombinant protein: 15 kD, the observed bands at 30 kD and 45 kD are the dimer and trimer, respectively.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling TSLP with TSLP Polyclonal Antibody (Product # PA5-20320) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling TSLP with TSLP Polyclonal Antibody (Product # PA5-20320) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using TSLP Polyclonal Antibody (Product # PA5-20320) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using TSLP Polyclonal Antibody (Product # PA5-20320) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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