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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
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- Product number
- AF1476 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat FABP5/E-FABP Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse FABP5/E-FABP in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse FABP4 and recombinant human (rh) FABP3 is observed and less than 1% cross-reactivity with recombinant rat (rr) FABP2 and rrFABP1 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q05816- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin.
Adipose Fatty Acid Binding Protein Promotes Saturated Fatty Acid-Induced Macrophage Cell Death through Enhancing Ceramide Production.
BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.
Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor β/δ (PPARβ/δ) in the brain.
Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-β responses in tumor-associated macrophages.
Retinoic acid induces neurogenesis by activating both retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor β/δ (PPARβ/δ).
Proteomic analysis of proteins differentially expressed in uterine lymphocytes obtained from wild-type and NOD mice.
All-trans-retinoic acid represses obesity and insulin resistance by activating both peroxisome proliferation-activated receptor beta/delta and retinoic acid receptor.
Helper-dependent adenovirus-mediated short hairpin RNA expression in the liver activates the interferon response.
Singh K, Camera E, Krug L, Basu A, Pandey RK, Munir S, Wlaschek M, Kochanek S, Schorpp-Kistner M, Picardo M, Angel P, Niemann C, Maity P, Scharffetter-Kochanek K
Nature communications 2018 Aug 24;9(1):3425
Nature communications 2018 Aug 24;9(1):3425
Adipose Fatty Acid Binding Protein Promotes Saturated Fatty Acid-Induced Macrophage Cell Death through Enhancing Ceramide Production.
Zhang Y, Rao E, Zeng J, Hao J, Sun Y, Liu S, Sauter ER, Bernlohr DA, Cleary MP, Suttles J, Li B
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 15;198(2):798-807
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 15;198(2):798-807
BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.
Kretzschmar K, Cottle DL, Donati G, Chiang MF, Quist SR, Gollnick HP, Natsuga K, Lin KI, Watt FM
Stem cell reports 2014 Oct 14;3(4):620-33
Stem cell reports 2014 Oct 14;3(4):620-33
Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor β/δ (PPARβ/δ) in the brain.
Yu S, Levi L, Casadesus G, Kunos G, Noy N
The Journal of biological chemistry 2014 May 2;289(18):12748-58
The Journal of biological chemistry 2014 May 2;289(18):12748-58
Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-β responses in tumor-associated macrophages.
Zhang Y, Sun Y, Rao E, Yan F, Li Q, Zhang Y, Silverstein KA, Liu S, Sauter E, Cleary MP, Li B
Cancer research 2014 Jun 1;74(11):2986-98
Cancer research 2014 Jun 1;74(11):2986-98
Retinoic acid induces neurogenesis by activating both retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor β/δ (PPARβ/δ).
Yu S, Levi L, Siegel R, Noy N
The Journal of biological chemistry 2012 Dec 7;287(50):42195-205
The Journal of biological chemistry 2012 Dec 7;287(50):42195-205
Proteomic analysis of proteins differentially expressed in uterine lymphocytes obtained from wild-type and NOD mice.
Li C, Wang W, Wang H, Zhong Y, Di J, Lin Y
Journal of cellular biochemistry 2009 Oct 1;108(2):447-57
Journal of cellular biochemistry 2009 Oct 1;108(2):447-57
All-trans-retinoic acid represses obesity and insulin resistance by activating both peroxisome proliferation-activated receptor beta/delta and retinoic acid receptor.
Berry DC, Noy N
Molecular and cellular biology 2009 Jun;29(12):3286-96
Molecular and cellular biology 2009 Jun;29(12):3286-96
Helper-dependent adenovirus-mediated short hairpin RNA expression in the liver activates the interferon response.
Witting SR, Brown M, Saxena R, Nabinger S, Morral N
The Journal of biological chemistry 2008 Jan 25;283(4):2120-8
The Journal of biological chemistry 2008 Jan 25;283(4):2120-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat FABP5/E-FABP by Western Blot. Western blot shows lysates of human placenta tissue, mouse liver tissue, rat brain tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/ Rat FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1476) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP5/ E-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
