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Western blot
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-24029 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FABP5 Monoclonal Antibody (311215)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, no cross-reactivity with recombinant human FABP1, 2, 3, 4, 6, 7, or 9 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 311215
- Vial size
- 100 μg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Fatty-acid binding protein 5 modulates the SAR1 GTPase cycle and enhances budding of large COPII cargoes.
Melville D, Gorur A, Schekman R
Molecular biology of the cell 2019 Feb 1;30(3):387-399
Molecular biology of the cell 2019 Feb 1;30(3):387-399
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of FABP5 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-human FABP5 Monoclonal Antibody (Product # MA5-24029) at 10 µg/mL for 3 hours at room temperature. Cells were stained using a 557-conjugated Anti-Rat IgG Secondary Antibody (yello and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 6: FABP5 affects collagen budding and secretion. (A) Immunoblot of response of traditional COPII cargoes ERGIC53 and SEC22B to FABP5 added to budding reactions with HeLa donor membranes. Ribophorin used as a marker of ER contamination. (B) Schematic of OptiPrep gradient flotation of budding reaction using IMR90 cell donor membrane. (C) Immunoblot of cargoes from budding reaction, with ribophorin as a control for ER contamination. (D) Immunoblot of type I collagen in media and procollagen in cells after 30 min of ascorbic acid treatment in IMR90 and U2OS cells after siRNA knockdown of FABP5. Actin was used as loading control. (E) Quantification by Student's two -tailed t test; p values indicated ( n = 3). (F) Immunoblot of type I collagen in media and procollagen in cells after 30 min of ascorbic acid treatment in IMR90 after overnight doxycycline treatment to overexpress FABP5. Actin was used as loading control. (G) Quantification by Student's two -tailed t test; p values indicated ( n = 4). (H) Immunofluorescently labeled IMR90 cells. Nuclei are stained with DAPI. (I) Quantification of collagen and FABP5 levels observed in 100 cells.
