Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 11-0019-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD1a Monoclonal Antibody (HI149), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic. Similar to MHC class I, CD1a associates with the beta2-microglobulin and is thought to play a role in antigen presentation. Applications Reported: The HI149 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI149 antibody has been pre-titrated and tested by flow cytometric analysis of MOLT-4 cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- HI149
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references In vitro OP9-DL1 co-culture and subsequent maturation in the presence of IL-21 generates tumor antigen-specific T cells with a favorable less-differentiated phenotype and enhanced functionality.
Biological Therapy in Inflammatory Bowel Disease Patients Partly Restores Intestinal Innate Lymphoid Cell Subtype Equilibrium.
Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.
Leishmania infantum and Leishmania braziliensis: Differences and Similarities to Evade the Innate Immune System.
Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.
MicroRNAs modulate the noncanonical transcription factor NF-kappaB pathway by regulating expression of the kinase IKKalpha during macrophage differentiation.
Dendritic cells express hematopoietic prostaglandin D synthase and function as a source of prostaglandin D2 in the skin.
CCL11 blocks IL-4 and GM-CSF signaling in hematopoietic cells and hinders dendritic cell differentiation via suppressor of cytokine signaling expression.
An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice.
Bonte S, de Munter S, Billiet L, Goetgeluk G, Ingels J, Jansen H, Pille M, de Cock L, Weening K, Taghon T, Leclercq G, Vandekerckhove B, Kerre T
Oncoimmunology 2021;10(1):1954800
Oncoimmunology 2021;10(1):1954800
Biological Therapy in Inflammatory Bowel Disease Patients Partly Restores Intestinal Innate Lymphoid Cell Subtype Equilibrium.
Creyns B, Jacobs I, Verstockt B, Cremer J, Ballet V, Vandecasteele R, Vanuytsel T, Ferrante M, Vermeire S, Van Assche G, Ceuppens JL, Breynaert C
Frontiers in immunology 2020;11:1847
Frontiers in immunology 2020;11:1847
Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.
Lopes DM, Oliveira SC, Page B, Carvalho LP, Carvalho EM, Cardoso LS
Frontiers in immunology 2018;9:3122
Frontiers in immunology 2018;9:3122
Leishmania infantum and Leishmania braziliensis: Differences and Similarities to Evade the Innate Immune System.
Falcão Sde A, Jaramillo TM, Ferreira LG, Bernardes DM, Santana JM, Favali CB
Frontiers in immunology 2016;7:287
Frontiers in immunology 2016;7:287
Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.
Lopes DM, Fernandes JS, Cardoso TM, Bafica AM, Oliveira SC, Carvalho EM, Araujo MI, Cardoso LS
BioMed research international 2014;2014:743069
BioMed research international 2014;2014:743069
MicroRNAs modulate the noncanonical transcription factor NF-kappaB pathway by regulating expression of the kinase IKKalpha during macrophage differentiation.
Li T, Morgan MJ, Choksi S, Zhang Y, Kim YS, Liu ZG
Nature immunology 2010 Sep;11(9):799-805
Nature immunology 2010 Sep;11(9):799-805
Dendritic cells express hematopoietic prostaglandin D synthase and function as a source of prostaglandin D2 in the skin.
Shimura C, Satoh T, Igawa K, Aritake K, Urade Y, Nakamura M, Yokozeki H
The American journal of pathology 2010 Jan;176(1):227-37
The American journal of pathology 2010 Jan;176(1):227-37
CCL11 blocks IL-4 and GM-CSF signaling in hematopoietic cells and hinders dendritic cell differentiation via suppressor of cytokine signaling expression.
Stevenson NJ, Addley MR, Ryan EJ, Boyd CR, Carroll HP, Paunovic V, Bursill CA, Miller HC, Channon KM, McClurg AE, Armstrong MA, Coulter WA, Greaves DR, Johnston JA
Journal of leukocyte biology 2009 Feb;85(2):289-97
Journal of leukocyte biology 2009 Feb;85(2):289-97
An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice.
Tieu BC, Lee C, Sun H, Lejeune W, Recinos A 3rd, Ju X, Spratt H, Guo DC, Milewicz D, Tilton RG, Brasier AR
The Journal of clinical investigation 2009 Dec;119(12):3637-51
The Journal of clinical investigation 2009 Dec;119(12):3637-51
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of MOLT-4 with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD1a FITC (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 660, were used for analysis.
- Conjugate
- Green dye