Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-0019-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD1a Monoclonal Antibody (HI149), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic. Similar to MHC class I, CD1a associates with the beta2-microglobulin and is thought to play a role in antigen presentation. Applications Reported: The HI149 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. Applications Tested: This HI149 antibody has been tested by flow cytometric analysis of human MOLT-4 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HI149
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Opposing chemokine gradients control human thymocyte migration in situ.
Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.
Ligand of scavenger receptor class A indirectly induces maturation of human blood dendritic cells via production of tumor necrosis factor-alpha.
Halkias J, Melichar HJ, Taylor KT, Ross JO, Yen B, Cooper SB, Winoto A, Robey EA
The Journal of clinical investigation 2013 May;123(5):2131-42
The Journal of clinical investigation 2013 May;123(5):2131-42
Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.
Freire T, Zhang X, Dériaud E, Ganneau C, Vichier-Guerre S, Azria E, Launay O, Lo-Man R, Bay S, Leclerc C
Blood 2010 Nov 4;116(18):3526-36
Blood 2010 Nov 4;116(18):3526-36
Ligand of scavenger receptor class A indirectly induces maturation of human blood dendritic cells via production of tumor necrosis factor-alpha.
Jin JO, Park HY, Xu Q, Park JI, Zvyagintseva T, Stonik VA, Kwak JY
Blood 2009 Jun 4;113(23):5839-47
Blood 2009 Jun 4;113(23):5839-47
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of MOLT-4 cell line with 0.5 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or Anti-Human CD1a Purified (filled histogram) followed by Anti-Mouse FITC. Total viable cells were used for the analysis.