NBP1-89850

antibody from Novus Biologicals

Targeting: RPAP3 FLJ21908, hSpagh, spag, Tah1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-89850

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-89850, RRID:AB_11049425

Product name

Rabbit Polyclonal FLJ21908 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human FLJ21908 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FLJ21908 Antibody [NBP1-89850] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FLJ21908 Antibody [NBP1-89850] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FLJ21908 Antibody [NBP1-89850] - Analysis using Anti-RPAP3 antibody NBP1-89850 (A) shows similar pattern to independent antibody NBP1-89849 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human fallopian tube.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human cerebral cortex.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human cerebral cortex, colon, fallopian tube and testis using Anti-RPAP3 antibody NBP1-89850 (A) shows similar protein distribution across tissues to independent antibody NBP1-89849 (B).