Explore
Validate
LearnPA5-58449
antibody from Invitrogen Antibodies
Targeting: RNF111
ARK, Arkadia, DKFZP761D081, FLJ38008
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-58449 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF111 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GTPSDEDNDS SFSDCLSSPS SSLHFGDSDT VTSDEDKEVS VRHSQTILNA KSRSHSARSH KWPRTETESV SGLLMKRPCL HGSSLRRLPC Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 86%.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references RNF111-facilitated neddylation potentiates cGAS-mediated antiviral innate immune response.
Li C, Zhang L, Qian D, Cheng M, Hu H, Hong Z, Cui Y, Yu H, Wang Q, Zhu J, Meng W, Xu JF, Sun Y, Zhang P, Wang C
PLoS pathogens 2021 Mar;17(3):e1009401
PLoS pathogens 2021 Mar;17(3):e1009401
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RNF111 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a RNF111 Polyclonal Antibody (Product # PA5-58449).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RNF111 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a RNF111 Polyclonal Antibody (Product # PA5-58449).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RNF111 in human cell line A-431 shows positivity in nucleoli & cytoplasm. Samples were probed using a RNF111 Polyclonal Antibody (Product # PA5-58449).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF111 in human placenta using RNF111 Polyclonal Antibody (Product # PA5-58449) shows strong granular cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF111 in human liver using RNF111 Polyclonal Antibody (Product # PA5-58449) shows moderate granular cytoplasmic positivity in hepatocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF111 in human lymph node using RNF111 Polyclonal Antibody (Product # PA5-58449) shows moderate to strong granular cytoplasmic positivity in lymphoid cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF111 in human rectum using RNF111 Polyclonal Antibody (Product # PA5-58449) shows moderate to strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF111 in human testis using RNF111 Polyclonal Antibody (Product # PA5-58449) shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.
