H00054778-M05

antibody from Novus Biologicals

Targeting: RNF111 ARK, Arkadia, DKFZP761D081, FLJ38008

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00054778-M05

Provider

Novus Biologicals

Proper citation

Novus Cat#H00054778-M05, RRID:AB_2285381

Product name

Mouse Monoclonal RNF111 Antibody

Antibody type

Monoclonal

Description

IgG purified. RNF111 - ring finger protein 111

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Arkadia (RING Finger Protein 111) Mediates Sumoylation-Dependent Stabilization of Nrf2 Through K48-Linked Ubiquitination.

McIntosh DJ, Walters TS, Arinze IJ, Davis J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(1):418-430

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c-Myc is targeted to the proteasome for degradation in a SUMOylation-dependent manner, regulated by PIAS1, SENP7 and RNF4.

González-Prieto R, Cuijpers SA, Kumar R, Hendriks IA, Vertegaal AC
Cell cycle (Georgetown, Tex.) 2015;14(12):1859-72

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SUMO and ubiquitin-dependent XPC exchange drives nucleotide excision repair.

van Cuijk L, van Belle GJ, Turkyilmaz Y, Poulsen SL, Janssens RC, Theil AF, Sabatella M, Lans H, Mailand N, Houtsmuller AB, Vermeulen W, Marteijn JA
Nature communications 2015 Jul 7;6:7499

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RNF111-dependent neddylation activates DNA damage-induced ubiquitination.

Ma T, Chen Y, Zhang F, Yang CY, Wang S, Yu X
Molecular cell 2013 Mar 7;49(5):897-907

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Arkadia, a novel SUMO-targeted ubiquitin ligase involved in PML degradation.

Erker Y, Neyret-Kahn H, Seeler JS, Dejean A, Atfi A, Levy L
Molecular and cellular biology 2013 Jun;33(11):2163-77

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RNF111/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage response.

Poulsen SL, Hansen RK, Wagner SA, van Cuijk L, van Belle GJ, Streicher W, Wikström M, Choudhary C, Houtsmuller AB, Marteijn JA, Bekker-Jensen S, Mailand N
The Journal of cell biology 2013 Jun 10;201(6):797-807

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RB1CC1 protein positively regulates transforming growth factor-beta signaling through the modulation of Arkadia E3 ubiquitin ligase activity.

Koinuma D, Shinozaki M, Nagano Y, Ikushima H, Horiguchi K, Goto K, Chano T, Saitoh M, Imamura T, Miyazono K, Miyazawa K
The Journal of biological chemistry 2011 Sep 16;286(37):32502-12

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Efficient TGF-β/SMAD signaling in human melanoma cells associated with high c-SKI/SnoN expression.

Javelaud D, van Kempen L, Alexaki VI, Le Scolan E, Luo K, Mauviel A
Molecular cancer 2011 Jan 6;10(1):2

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Context-dependent regulation of the expression of c-Ski protein by Arkadia in human cancer cells.

Nagano Y, Koinuma D, Miyazawa K, Miyazono K
Journal of biochemistry 2010 Apr;147(4):545-54

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Overexpression of SnoN/SkiL, amplified at the 3q26.2 locus, in ovarian cancers: a role in ovarian pathogenesis.

Nanjundan M, Cheng KW, Zhang F, Lahad J, Kuo WL, Schmandt R, Smith-McCune K, Fishman D, Gray JW, Mills GB
Molecular oncology 2008 Aug;2(2):164-81

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Arkadia activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.

Levy L, Howell M, Das D, Harkin S, Episkopou V, Hill CS
Molecular and cellular biology 2007 Sep;27(17):6068-83

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Western blot analysis of RNF111 over-expressed 293 cell line, cotransfected with RNF111 Validated Chimera RNAi or non-transfected control. Blot probed with H00054778-M05. GAPDH (36.1 kDa) used as loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of RNF111 expression in transfected 293T cell line by RNF111 monoclonal antibody (M05), clone 1C4. Lane 1: RNF111 transfected lysatE (107.8 KDa). Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: RNF111 Antibody (1C4) [H00054778-M05] - Detection limit for recombinant GST tagged RNF111 is 0.03 ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of monoclonal antibody to RNF111 on HeLa cell. Antibody concentration 10 ug/ml