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- Antibody Data
- Antigen structure
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- Immunocytochemistry [2]
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- Product number
- MA5-18110 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG4D Monoclonal Antibody (222CT15.4.1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 222CT15.4.1
- Vial size
- 400 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The insufficiency of ATG4A in macroautophagy.
Nguyen N, Olivas TJ, Mires A, Jin J, Yu S, Luan L, Nag S, Kauffman KJ, Melia TJ
The Journal of biological chemistry 2020 Sep 25;295(39):13584-13600
The Journal of biological chemistry 2020 Sep 25;295(39):13584-13600
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATG4D in untreated U-251 cells (left) and U-251 cells treated with Chloroquine (50 uM, 16h) (right) using an ATG4D monoclonal antibody (Product # MA5-18110) at a dilution of 1:25, followed by detection using a Dylight 488-conjugated secondary antibody at a dilution of 1:400. Nuclei were stained with Dapi (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ATG4D in U251 cells. Samples were incubated with ATG4D monoclonal antibody (Product # MA5-18110) using a dilution of 1:25 followed by Dylight Fluor 488-conjugated goat anti-mouse lgG at a dilution of 1:400 (green). Cells (right) were treated with Chloroquine (50 μM, 16hr). DAPI was used to stain the cell nuclear (blue).
