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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA5-18110 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG4D Monoclonal Antibody (222CT15.4.1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 222CT15.4.1
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The insufficiency of ATG4A in macroautophagy.
Nguyen N, Olivas TJ, Mires A, Jin J, Yu S, Luan L, Nag S, Kauffman KJ, Melia TJ
The Journal of biological chemistry 2020 Sep 25;295(39):13584-13600
The Journal of biological chemistry 2020 Sep 25;295(39):13584-13600
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG4D in HL-60 cell lysate (35 µg/lane) using an ATG4D monoclonal antibody (Product # MA5-18110).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATG4D in untreated U-251 cells (left) and U-251 cells treated with Chloroquine (50 uM, 16h) (right) using an ATG4D monoclonal antibody (Product # MA5-18110) at a dilution of 1:25, followed by detection using a Dylight 488-conjugated secondary antibody at a dilution of 1:400. Nuclei were stained with Dapi (blue).
