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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F54718 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Autophagin 4D Antibody / ATG4D
- Antibody type
- Polyclonal
- Description
- This highly specific Autophagin 4D antibody is suitable for use in Immunohistochemistry/Immunofluorescence/Western blot applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Autophagin 4D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Y79 cell lysate with Autophagin 4D antibody. Expected molecular weight: 53-56 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) monkey COS7, 2) human HEK293, 3) MEF (mouse embryonic fibroblasts) and 4) human HeLa cell lysate with Autophagin 4D antibody. Expected molecular weight: 53-56 kDa. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with Autophagin 4D antibody (green) and Hoechst 33342 nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human cancer tissue with Autophagin 4D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
