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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-28363 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG4D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Predicted reactivity: Mouse (95%), Rat (95%), Pig (97%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ATG4D was performed in 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D Polyclonal Antibody (Product # PA5-28363) diluted at 1:200. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ATG4D was performed in 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D Polyclonal Antibody (Product # PA5-28363) diluted at 1:200. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ATG4D was performed in paraffin-embedded mouse ovary tissue using ATG4D Polyclonal Antibody (Product # PA5-28363) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ATG4D was performed in paraffin-embedded rat muscle tissue using ATG4D Polyclonal Antibody (Product # PA5-28363) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS xenograft , using ATG4D (Product # PA5-28363) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
