14643-1-AP

antibody from Proteintech Group

Targeting: REEP5 C5orf18, D5S346, DP1, POB16, TB2, Yip2e

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

14643-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14643-1-AP, RRID:AB_2178440

Product name

REEP5 antibody

Antibody type

Polyclonal

Description

KD/KO validated REEP5 antibody (Cat. #14643-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, Harper JW
Nature cell biology 2024 Mar;26(3):378-392

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ATG14 targets lipid droplets and acts as an autophagic receptor for syntaxin18-regulated lipid droplet turnover.

Yuan Z, Cai K, Li J, Chen R, Zhang F, Tan X, Jiu Y, Chang H, Hu B, Zhang W, Ding B
Nature communications 2024 Jan 20;15(1):631

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Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis.

Zhang W, Zhang Z, Xiang Y, Gu DD, Chen J, Chen Y, Zhai S, Liu Y, Jiang T, Liu C, He B, Yan M, Wang Z, Xu J, Cao YL, Deng B, Zeng D, Lei J, Zhuo J, Lei X, Long Z, Jin B, Chen T, Li D, Shen Y, Hu J, Gao S, Liu Q
Nature structural & molecular biology 2024 Feb;31(2):219-231

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Integrated proteomics reveals autophagy landscape and an autophagy receptor controlling PKA-RI complex homeostasis in neurons.

Zhou X, Lee YK, Li X, Kim H, Sanchez-Priego C, Han X, Tan H, Zhou S, Fu Y, Purtell K, Wang Q, Holstein GR, Tang B, Peng J, Yang N, Yue Z
Nature communications 2024 Apr 10;15(1):3113

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Spatacsin regulates directionality of lysosome trafficking by promoting the degradation of its partner AP5Z1.

Pierga A, Matusiak R, Cauhapé M, Branchu J, Danglot L, Boutry M, Darios F
PLoS biology 2023 Oct;21(10):e3002337

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Combinatorial selective ER-phagy remodels the ER during neurogenesis.

Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, Harper JW
bioRxiv : the preprint server for biology 2023 Nov 10;

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Pathological characteristics of axons and proteome patterns in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency.

Le W, Wang P, Al-Nusaif M, Zhang J, Yang H, Yang Y, Kim K, Li S, Liu C, Cai H
Research square 2023 May 18;

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Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.

Foronda H, Fu Y, Covarrubias-Pinto A, Bocker HT, González A, Seemann E, Franzka P, Bock A, Bhaskara RM, Liebmann L, Hoffmann ME, Katona I, Koch N, Weis J, Kurth I, Gleeson JG, Reggiori F, Hummer G, Kessels MM, Qualmann B, Mari M, Dikić I, Hübner CA
Nature 2023 Jun;618(7964):402-410

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Ubiquitination regulates ER-phagy and remodelling of endoplasmic reticulum.

González A, Covarrubias-Pinto A, Bhaskara RM, Glogger M, Kuncha SK, Xavier A, Seemann E, Misra M, Hoffmann ME, Bräuning B, Balakrishnan A, Qualmann B, Dötsch V, Schulman BA, Kessels MM, Hübner CA, Heilemann M, Hummer G, Dikić I
Nature 2023 Jun;618(7964):394-401

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Identification of several inflammation-related genes based on bioinformatics and experiments.

Wang S, Zhang Z, Liang J, Li K, Bo L, Zhan H, Hong X, Hu J, Yang Qian L, Liu X, Zhang B
International immunopharmacology 2023 Aug;121:110409

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Coronavirus subverts ER-phagy by hijacking FAM134B and ATL3 into p62 condensates to facilitate viral replication.

Tan X, Cai K, Li J, Yuan Z, Chen R, Xiao H, Xu C, Hu B, Qin Y, Ding B
Cell reports 2023 Apr 25;42(4):112286

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Proteomics Identifies Substrates and a Novel Component in hSnd2-Dependent ER Protein Targeting.

Tirincsi A, O'Keefe S, Nguyen D, Sicking M, Dudek J, Förster F, Jung M, Hadzibeganovic D, Helms V, High S, Zimmermann R, Lang S
Cells 2022 Sep 19;11(18)

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Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia.

Byrne DJ, Garcia-Pardo ME, Cole NB, Batnasan B, Heneghan S, Sohail A, Blackstone C, O'Sullivan NC
Acta neuropathologica communications 2022 Mar 28;10(1):40

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ER proteins decipher the tubulin code to regulate organelle distribution.

Zheng P, Obara CJ, Szczesna E, Nixon-Abell J, Mahalingan KK, Roll-Mecak A, Lippincott-Schwartz J, Blackstone C
Nature 2022 Jan;601(7891):132-138

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Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Zhu PP, Hung HF, Batchenkova N, Nixon-Abell J, Henderson J, Zheng P, Renvoisé B, Pang S, Xu CS, Saalfeld S, Funke J, Xie Y, Svara F, Hess HF, Blackstone C
Human molecular genetics 2022 Aug 23;31(16):2779-2795

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ATF4 links ER stress with reticulophagy in glioblastoma cells.

Zielke S, Kardo S, Zein L, Mari M, Covarrubias-Pinto A, Kinzler MN, Meyer N, Stolz A, Fulda S, Reggiori F, Kögel D, van Wijk S
Autophagy 2021 Sep;17(9):2432-2448

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A novel automated image analysis pipeline for quantifying morphological changes to the endoplasmic reticulum in cultured human cells.

Garcia-Pardo ME, Simpson JC, O'Sullivan NC
BMC bioinformatics 2021 Sep 8;22(1):427

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FIT2 organizes lipid droplet biogenesis with ER tubule-forming proteins and septins.

Chen F, Yan B, Ren J, Lyu R, Wu Y, Guo Y, Li D, Zhang H, Hu J
The Journal of cell biology 2021 May 3;220(5)

---

Mechanism of membrane-curvature generation by ER-tubule shaping proteins.

Wang N, Clark LD, Gao Y, Kozlov MM, Shemesh T, Rapoport TA
Nature communications 2021 Jan 25;12(1):568

---

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Wang X, Wang H, Xu B, Huang D, Nie C, Pu L, Zajac GJM, Yan H, Zhao J, Shi F, Emmer BT, Lu J, Wang R, Dong X, Dai J, Zhou W, Wang C, Gao G, Wang Y, Willer C, Lu X, Zhu Y, Chen XW
Cell metabolism 2021 Feb 2;33(2):350-366.e7

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A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.

Cheng YS, Zhang T, Ma X, Pratuangtham S, Zhang GC, Ondrus AA, Mafi A, Lomenick B, Jones JJ, Ondrus AE
Nature chemical biology 2021 Dec;17(12):1271-1280

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Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190.

Dickson P, Abegg D, Vinogradova E, Takaya J, An H, Simanski S, Cravatt BF, Adibekian A, Kodadek T
Cell chemical biology 2020 Nov 19;27(11):1371-1382.e6

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DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.

Tian X, Zheng P, Zhou C, Wang X, Ma H, Ma W, Zhou X, Teng J, Chen J
Autophagy 2020 May;16(5):797-810

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Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Yamanaka T, Nishiyama R, Shimogori T, Nukina N
Scientific reports 2020 May 6;10(1):7610

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Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes.

Callaghan NI, Lee SH, Hadipour-Lakmehsari S, Lee XA, Ahsan Siraj M, Driouchi A, Yip CM, Husain M, Simmons CA, Gramolini AO
Communications biology 2020 May 11;3(1):229

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A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.

Liang JR, Lingeman E, Luong T, Ahmed S, Muhar M, Nguyen T, Olzmann JA, Corn JE
Cell 2020 Mar 19;180(6):1160-1177.e20

---

REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects.

Lee SH, Hadipour-Lakmehsari S, Murthy HR, Gibb N, Miyake T, Teng ACT, Cosme J, Yu JC, Moon M, Lim S, Wong V, Liu P, Billia F, Fernandez-Gonzalez R, Stagljar I, Sharma P, Kislinger T, Scott IC, Gramolini AO
Nature communications 2020 Feb 19;11(1):965

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Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.

Sharoar MG, Hu X, Ma XM, Zhu X, Yan R
Molecular psychiatry 2019 Sep;24(9):1369-1382

---

ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.

Chen Q, Xiao Y, Chai P, Zheng P, Teng J, Chen J
Current biology : CB 2019 Mar 4;29(5):846-855.e6

---

TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.

An H, Ordureau A, Paulo JA, Shoemaker CJ, Denic V, Harper JW
Molecular cell 2019 Jun 6;74(5):891-908.e10

---

REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum during mitosis.

Kumar D, Golchoubian B, Belevich I, Jokitalo E, Schlaitz AL
Molecular biology of the cell 2019 Jun 1;30(12):1377-1389

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Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus.

Li L, Wu J, Cao X, Zhou J, Yin S, Yang S, Feng Q, Du P, Liu Y, Shang Y, Liu Y, Liu X
Research in veterinary science 2019 Aug;125:195-204

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Combination anti-Aβ treatment maximizes cognitive recovery and rebalances mTOR signaling in APP mice.

Chiang ACA, Fowler SW, Savjani RR, Hilsenbeck SG, Wallace CE, Cirrito JR, Das P, Jankowsky JL
The Journal of experimental medicine 2018 May 7;215(5):1349-1364

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DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Zheng P, Chen Q, Tian X, Qian N, Chai P, Liu B, Hu J, Blackstone C, Zhu D, Teng J, Chen J
Cell research 2018 Aug;28(8):833-854

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Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae.

Wang X, Li S, Wang H, Shui W, Hu J
eLife 2017 Mar 13;6

---

Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.

Grumati P, Morozzi G, Hölper S, Mari M, Harwardt MI, Yan R, Müller S, Reggiori F, Heilemann M, Dikic I
eLife 2017 Jun 15;6

---

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Sharoar MG, Shi Q, Ge Y, He W, Hu X, Perry G, Zhu X, Yan R
Molecular psychiatry 2016 Sep;21(9):1263-71

---

Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.

Renvoisé B, Malone B, Falgairolle M, Munasinghe J, Stadler J, Sibilla C, Park SH, Blackstone C
Human molecular genetics 2016 Dec 1;25(23):5111-5125

---

Molecular Mechanisms of Disease Pathogenesis Differ in Krabbe Disease Variants.

Spratley SJ, Hill CH, Viuff AH, Edgar JR, Skjødt K, Deane JE
Traffic (Copenhagen, Denmark) 2016 Aug;17(8):908-22

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ER network formation and membrane fusion by atlastin1/SPG3A disease variants.

Ulengin I, Park JJ, Lee TH
Molecular biology of the cell 2015 May 1;26(9):1616-28

---

A high-content screening microscopy approach to dissect the role of Rab proteins in Golgi-to-ER retrograde trafficking.

Galea G, Bexiga MG, Panarella A, O'Neill ED, Simpson JC
Journal of cell science 2015 Jul 1;128(13):2339-49

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Spastin binds to lipid droplets and affects lipid metabolism.

Papadopoulos C, Orso G, Mancuso G, Herholz M, Gumeni S, Tadepalle N, Jüngst C, Tzschichholz A, Schauss A, Höning S, Trifunovic A, Daga A, Rugarli EI
PLoS genetics 2015 Apr;11(4):e1005149

---

Pharmacologic rescue of axon growth defects in a human iPSC model of hereditary spastic paraplegia SPG3A.

Zhu PP, Denton KR, Pierson TM, Li XJ, Blackstone C
Human molecular genetics 2014 Nov 1;23(21):5638-48

---

Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.

Chang J, Lee S, Blackstone C
Proceedings of the National Academy of Sciences of the United States of America 2013 Sep 10;110(37):14954-9

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human liver tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP(REEP5 antibody) at dilution of 1:500

Sample type

tissue

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-REEP5 (IP:14643-1-AP, 3ug; Detection:14643-1-AP 1:1000) with HeLa cells lysate 2400ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 14643-1-AP(REEP5 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The REEP5 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human REEP5. This antibody recognizes human,mouse,rat antigen. The REEP5 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.

Appendix

14643-1-AP.pdf

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