H00003696-M01

antibody from Abnova Corporation

Targeting: ITGB8

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003696-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003696-M01, RRID:AB_565871

Product name

ITGB8 monoclonal antibody (M01), clone 2B4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ITGB8.

Antigen sequence

VENQVQGIYFNITAICPDGSRKPGMEGCRNVTSND
EVLFNVTVTMKKCDVTGGKNYAIIKPIGFNETAKI
HIHRNCSCQCEDNRGPKGKCVDETFLDSKCFQCDE
NKCHFDE


Isotype

IgG

Antibody clone number

2B4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

β5 integrin is the major contributor to the αVintegrin-mediated blockade of HIV-1 replication.

Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Esté JA
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):464-70

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ITGB8 expression in transfected 293T cell line by ITGB8 monoclonal antibody (M01), clone 2B4.Lane 1: ITGB8 transfected lysate(85.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ITGB8 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of ITGB8 transfected lysate using anti-ITGB8 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ITGB8 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol