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- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-55692 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITGB8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AQHCVNSKGQ VCSGRGTCVC GRCECTDPRS IGRFCEHCPT CYTACKENWN CMQCLHPHNL SQAILDQCKT SCALMEQQHY Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 85%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ITGB8 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ITGB8 Polyclonal Antibody (Product # PA5-55692) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ITGB8 in human cervix, uterine using ITGB8 Polyclonal Antibody (Product # PA5-55692) shows strong membranous positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ITGB8 in human endometrium using ITGB8 Polyclonal Antibody (Product # PA5-55692) shows strong membranous positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ITGB8 in human kidney using ITGB8 Polyclonal Antibody (Product # PA5-55692) shows strong membranous positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ITGB8 in human liver using ITGB8 Polyclonal Antibody (Product # PA5-55692) shows no positivity in hepatocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ITGB8 in human retina using ITGB8 Polyclonal Antibody (Product # PA5-55692) shows moderate positivity in outer limiting membrane.
