PA5-55692

antibody from Invitrogen Antibodies

Targeting: ITGB8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55692

Provider

Invitrogen Antibodies

Product name

ITGB8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AQHCVNSKGQ VCSGRGTCVC GRCECTDPRS IGRFCEHCPT CYTACKENWN CMQCLHPHNL SQAILDQCKT SCALMEQQHY

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using ITGB8 Polyclonal Antibody (Product # PA5-55692) and a 86 kDa band corresponding to ITGB8 was observed in HaCa T and absent in LNCaP along with an uncharacterized band around ~30 kDa. Membrane extracts (30 µg lysate) of HaCa T (Lane 1) and LNCaP (Lane 2) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1500 (Product # A44115). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ITGB8 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ITGB8 Polyclonal Antibody (Product # PA5-55692) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ITGB8 in human retina using ITGB8 Polyclonal Antibody (Product # PA5-55692) shows moderate positivity in outer limiting membrane.