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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00003696-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003696-M01, RRID:AB_2129420
- Product name
- Mouse Monoclonal Integrin beta 8 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ITGB8 (2B4)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references β5 integrin is the major contributor to the αVintegrin-mediated blockade of HIV-1 replication.
Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Esté JA
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):464-70
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):464-70
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Integrin beta 8 Antibody (2B4) [H00003696-M01] - Analysis of ITGB8 expression in transfected 293T cell line by ITGB8 monoclonal antibody (M01), clone 2B4.Lane 1: ITGB8 transfected lysate(85.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Integrin beta 8 Antibody (2B4) [H00003696-M01] - Detection limit for recombinant GST tagged ITGB8 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Integrin beta 8 Antibody (2B4) [H00003696-M01] - Analysis of ITGB8 transfected lysate using anti-ITGB8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ITGB8 MaxPab rabbit polyclonal antibody.
