PA5-120270

antibody from Invitrogen Antibodies

Targeting: ACOT2 Mte1, ZAP128

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120270

Provider

Invitrogen Antibodies

Product name

ACOT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: 293T, LO2, U-87MG, Mouse kidney. The target is usually found in the following locations: Mitochondrion. Immunogen sequence: IVDMFGTGGG LLEYRASLLA GKGFAVMALA YYNYEDLPKT METLHLEYFE EAMNYLLSHP EVKGPGVGLL GISKGGELCL SMASFLKGIT AAVVINGSVA NVGGTLHYKG ETLPPVGVNR NRIKVTKDGY ADIVDVLNSP LEGPDQKSFI PVERAESTFL FLVGQDDHNW KSEFYANEAC KRLQAHGRRK PQIICYPETG HYIEPPYFPL CRASLHALVG SPIIWGGEPR AHAMAQVDAW KQLQTFFHKH LGGHEGTIPS KV

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.79 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ACOT2 in extracts of various cell lines. Samples were incubated with ACOT2 Polyclonal antibody (Product # PA5-120270) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ACOT2 in paraffin-embedded human appendix. Samples were incubated with ACOT2 Polyclonal antibody (Product # PA5-120270) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ACOT2 in paraffin-embedded mouse kidney. Samples were incubated with ACOT2 Polyclonal antibody (Product # PA5-120270) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ACOT2 in paraffin-embedded mouse kidney. Samples were incubated with ACOT2 Polyclonal antibody (Product # PA5-120270) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.