15858-1-AP

antibody from Proteintech Group

Targeting: SF3A1 Prp21, PRPF21, SAP114, SF3a120

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15858-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15858-1-AP, RRID:AB_2186354

Product name

SF3A1 antibody

Antibody type

Polyclonal

Description

SF3A1 antibody (Cat. #15858-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

The molecular axis hnRNPU/circKCNK2/EDC4/IL-11 aggravates osteolytic bone metastasis of RCC.

Wang Y, Zhao D, Lu J, Hou N, Wu Q, Zhou S, Xu J, Xue W, Cui W, Zheng J, Wang F, Zhai W
Oncogene 2025 Sep;44(36):3310-3332

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Hydroxysafflor yellow A alleviates ischemic myocardial injury by targeting SF3A1 to improve mitochondrial energy metabolism.

Wang BK, Wang R, Zhuo FF, Wang JK, Huang YL, Tu PF, Zeng KW, Li J
European journal of pharmacology 2025 Sep 5;1002:177789

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Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice.

Wang H, Zhang L, Chen X, Hong L, Zhao J, Qian W, Pham LK, Willard B, Li X, Bulek K, Li X
iScience 2025 Feb 21;28(2):111903

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Integrative identification of non-coding regulatory regions driving metastatic prostate cancer.

Woo BJ, Moussavi-Baygi R, Karner H, Karimzadeh M, Yousefi H, Lee S, Garcia K, Joshi T, Yin K, Navickas A, Gilbert LA, Wang B, Asgharian H, Feng FY, Goodarzi H
Cell reports 2024 Sep 24;43(9):114764

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ZC3H14 facilitates backsplicing by binding to exon-intron boundary and 3' UTR.

Li Q, Yang G, Ren B, Liu X, Tang LQ, Shi Q, Shan G, Wang X
Molecular cell 2024 Nov 21;84(22):4314-4333.e9

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Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations.

Zhou S, Lizarazo S, Mouli L, Chorghade S, Cheng R, Rajendra KC, Kalsotra A, Van Bortle K
bioRxiv : the preprint server for biology 2024 Jul 3;

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Integrative identification of non-coding regulatory regions driving metastatic prostate cancer.

Woo BJ, Moussavi-Baygi R, Karner H, Karimzadeh M, Garcia K, Joshi T, Yin K, Navickas A, Gilbert LA, Wang B, Asgharian H, Feng FY, Goodarzi H
bioRxiv : the preprint server for biology 2023 Jun 2;

---

LXR agonist inhibits inflammation through regulating MyD88 mRNA alternative splicing.

Li N, Li Y, Han X, Zhang J, Han J, Jiang X, Wang W, Xu Y, Xu Y, Fu Y, Si S
Frontiers in pharmacology 2022;13:973612

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SF3B1 association with chromatin determines splicing outcomes.

Kfir N, Lev-Maor G, Glaich O, Alajem A, Datta A, Sze SK, Meshorer E, Ast G
Cell reports 2015 Apr 28;11(4):618-29

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP(SF3A1 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SF3A1 antibody 15858-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 15858-1-AP(SF3A1 antibody) at dilution of 1:400 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 15858-1-AP(SF3A1 antibody) at dilution of 1:400 (under 40x lens)

Sample type

tissue