HPA000690

antibody from Atlas Antibodies

Targeting: SF3A1 Prp21, PRPF21, SAP114, SF3a120

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA000690

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000690, RRID:AB_1079933

Product name

Anti-SF3A1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human SF3A1, Gene description: splicing factor 3a, subunit 1, 120kDa, Alternative Gene Names: Prp21, PRPF21, SAP114, SF3a120, Validated applications: ICC, IHC, WB, Uniprot ID: Q15459, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

The nuclear splicing factor RNA binding motif 5 promotes caspase activation in human neuronal cells, and increases after traumatic brain injury in mice.

Jackson TC, Du L, Janesko-Feldman K, Vagni VA, Dezfulian C, Poloyac SM, Jackson EK, Clark RS, Kochanek PM
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2015 Mar 31;35(4):655-66

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SF3A1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Sample type

Human

Protocol

Protocol