14973-1-AP

antibody from Invitrogen Antibodies

Targeting: MTMR14 C3orf29, FLJ22405, FLJ90311, hEDTP, hJumpy

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14973-1-AP

Provider

Invitrogen Antibodies

Product name

MTMR14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LSIPYPGCE FFKEYKDRDY MAEGLIFNWK QDYVDAPLSI PDFLTHSLNI DWSQYQCWDL VQQTQNYLKL LLSLVNSDDD SGLLVHCISG WDRTPLFISL LRLSLWADGL IHTSLKPTEI LYLTVAYDWF LFGHMLVDRL SKGEEIFFFC FNFLKHITSE EFSALKTQRR KSLPARDGGF TLEDICMLRR KDRGSTTSLG SDFSLVMESS PGATGSFTYE AVELVPAGAP TQAAWRKSHS SSPQSVLWNR PQPSEDRLPS QQGLAEARSS SSSSSNHSDN FFRMGSSPLE VPKPRLAALS DRETRLQEVR SAFLAAYSST VGLRAVAPSP SGAIGGLLEQ FARGVGLRSI SSNAL (245-598 aa encoded by BC050626)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.34 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 14973-1-AP (MTMR14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 14973-1-AP (MTMR14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of MTMR14 antibody (14973-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR14 transfected HepG2 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14973-1-AP (MTMR14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14973-1-AP (MTMR14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using MTMR14 antibody 14973-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using MTMR14 antibody 14973-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug MTMR14 antibody (14973-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MTMR14 (IP:14973-1-AP, 4ug; Detection:14973-1-AP 1:500) with HeLa cells lysate 2000ug.